Study of phase separation immunoassay Progress_0.docVIP

 PAGE \* MERGEFORMAT 35 Study of phase separation immunoassay Progress [Abstract] phase separation immunoassay homogeneous through rapid response and simple phase separation both the homogeneous and heterogeneous immune immune advantages, the method is easy to automate. This article describes the separation of the basic principles of immunoassay, vector on the phase separation immunoassay, immobilized way markers, application and development were reviewed. 60 references. [Keywords:] Immunoassays, phase separation, environmentally sensitive, temperature-sensitive, pH sensitive, review 1 Introduction Phase separation immunoassay is an antigen (Antigen, Ag) or antibody (Antibody, Ab) coupled with a special carrier (which carrier will be small changes in the external environment as a result of precipitation and dissolution of the mutation, homogeneous solution incubated in the reaction of Ag and Ab, and then adjust the changes in the external environment, coupled with the carrier complex precipitation reactions, and raw materials were determined after separation. As a heterogeneous reaction, the reaction rate and degree of reaction were greater than the differences, but also because the same the same phase is separated after immunization were measured phase immunoassay sensitivity and considerable, so this method both the homogeneous and heterogeneous immunological advantages In 1986 Jones [1] first reported in summary form of phase separation immunoassay, followed by Hoffman and other research papers published in the first [2]. At present, phase separation immunoassay method has been widely applied. Immobilized Ag Ag and standard competitive immune reaction occurs with the tag Ab competition law, for example, the following steps: (1 Ag carrier fixed to the phase separation on markers linked with Ab, adjust the external environment, when the carrier for the immobilization of homogeneous Ag, standards of Ag, Ab labeled mixed reaction, (2 changes in