Survivin Gene Research.docVIP

 PAGE \* MERGEFORMAT 16 Survivin Gene Research [Keywords:] inhibitor of apoptosis protein; biological characteristics; tissue distribution Survivin is the inhibitor of apoptosis protein (Inhibitorofapoptosis, IAP) family, a new member [1], is found in the most powerful inhibitor of apoptosis, Survivin function of the complex, inhibits apoptosis, promoting cell transformation and to participate in cell mitosis, vascular formation and development of drug resistance of tumor cells and so on. At present, Survivin has become a research hotspot. 1 Survivin structure and biological characteristics of 1.1 Survivin structure in 1997, AMBROSINI et al [2] the use of effector cell protease receptor  1 (EPR  1) cDNA library in the human genome hybridization Survivin gene was first isolated due to prolonged cell survival, also known as Survivin or Survivin. The gene length of 15 KB, located in 17q25, containing four exons and three introns, Survivin gene encodes a product is composed of 142 amino acid protein, molecular weight of 16.2 KD. Human and Mouse protein homology of 84.3%. With the other members of the IAP family comparison, Survivin special structure, IAP family generally contain two ~ three series containing cysteine / histidine sequence, including a total of 70 amino acids of the baculovirus inhibitor of apoptosis protein Repeat series (baculovirus IAP repeat, BIR) and the hydroxyl end of the ring finger of molecular structure, in which the BIR molecules play a role in anti-apoptosis, while Survivin contains only a single BIR functional area by the Cys 46  Pro 47  Thr 483 amino acids Insertion sequence will be divided into two semi-Survivin, hydroxyl side does not contain ring finger, and substituting interwoven helical structure (coiled  coil), to distinguish it from other IAP family members. 1.2 Survivin expression in 1998, Li et al [3] with the cell division inhibitor blocked the cells at different times in cell proliferation (G1, S, G2 /