Antimicrobial Activity of Cannabis sativa L.英文文献资料.docVIP

Chinese Medicine, 2012, 3, 61-64 /10.4236/cm.2012.31010 Published Online March 2012 (http://www.SciRP.org/journal/cm) Antimicrobial Activity of Cannabis sativa L. Esra M. M. Ali , Aisha Z. I. Almagboul , Salwa M. E. Khogali , Umelkheir M. A. Gergeir 1 2 1 2 1 Department of Biochemistry, Nutrition and Toxicology, Central Veterinary Research Laboratories, Khartoum, Sudan 2 Department of Microbiology and Parasitology, Medicinal and Aromatic Plants Research Institute, National Centre for Research, Khartoum, Sudan Email: aalmagboul@ Received July 27, 2011; revised December 1, 2011; accepted December 15, 2011 ABSTRACT The oil of the seeds, petroleum ether and methanol extracts of the whole plant of Cannabis sativa belonging to the fam- ily Cannabinaceae were screened for their antimicrobial activity against two Gram positive organisms (Bacillus subtilis, Staphylococcus aureus), two Gram negative organisms (Escherichia coli, Pseudomonas aeruginosa) and two fungi namely Aspergillus niger and Candida albicans using the cup plate agar diffusion method. The oil of the seeds of Can- nabis sativa exerted pronounced antibacterial activity (21 - 28 mm) against Bacillus subtilis and Staphylococcus aureus, moderate activity (15 mm) against Escherichia coli and high activity (16 mm) against Pseudomonas aeruginosa and inactive against the two fungi tested. The petroleum ether extract of the whole plant exhibited pronounced antibacterial activity (23 - 28 mm) against both Bacillus subtilis and Staphylococcus aureus organisms, high activity (16 mm) against Escherichia coli and inactive against Pseudomonas aeruginosa and both fungi. The methanol extract of the whole plant showed also pronounced antibacterial activity (29 mm) against Bacillus subtilis, low activity (12 m