Antimicrobial Activity of Copper Alone and in Combination with Lactic Acid against Escherichia coli O157H7 in Laboratory Medium and on the Surface of Lettuce and Tomatoes英文文献资料.docVIP
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Antimicrobial Activity of Copper Alone and in Combination with Lactic Acid against Escherichia coli O157H7 in Laboratory Medium and on the Surface of Lettuce and Tomatoes英文文献资料
SAGE-HindawiAccesstoResearch
JournalofPathogens
Volume2011,ArticleID650968,9pages
doi:10.4061/2011/650968
ResearchArticle
AntimicrobialActivityofCopperAloneandinCombinationwith
LacticAcidagainstEscherichiacoliO157:H7in
LaboratoryMediumandontheSurfaceofLettuceandTomatoes
RabinGyawali,
1
SalamA.Ibrahim, SalmaH.AbuHasfa,
1 2
andYosefHaik
3
ShahnazQ.Smqadri,
3
1
FoodMicrobiologyandBiotechnologyLaboratory,NorthCarolinaAgriculturalandTechnicalStateUniversity,173CarverHall,
Greensboro,NC27411,USA
2
3
LFRDDepartment,ALCDRInstitute,CityofScienti?cResearchandTechnologyApplications,P.O.Box21934,Alexandria,Egypt
CenterofResearchExcellenceinNanobioscience,TheUniversityofNorthCarolinaGreensboro,Greensboro,NC27412,USA
CorrespondenceshouldbeaddressedtoSalamA.Ibrahim,ibrah001@
Received22June2011;Accepted22August2011
AcademicEditor:Hin-ChungWong
Copyright?2011RabinGyawalietal.ThisisanopenaccessarticledistributedundertheCreativeCommonsAttributionLicense,
whichpermitsunrestricteduse,distribution,andreproductioninanymedium,providedtheoriginalworkisproperlycited.
Theobjectiveofthisstudywastoevaluatethee?ectofcopperaloneandincombinationwithlacticacidagainstE.coliO157:H7
inlaboratorymediumandonthesurfaceoflettuceandtomatoes.FourstrainsofE.coliO157:H7wereindividuallyinoculated
intoBHIbrothcontainingdi?erentconcentrationsofcopper(5,10,20,and40ppm,w/v),lacticacid(0.1and0.2%,v/v),and
theircombinations.Afterincubation,aliquotsof1mLfromeachsamplewerewithdrawnandplatedonBHIagartodetermine
thebacterialpopulation.Signi?cantgrowthinhibition(P0.05)wasobservedwithacombinationtreatmentofcopper(40ppm)
andlacticacid(0.2%).ThepopulationofE.coliO157:H7wasreducedby3.93and3.39logonthesurfaceoflettuceandtomato
samples,respectively,whentreatedwiththesamecombination.Thisindicatesthatcombinationofcopperandlacticacidcouldbe
usedasane?ectivesolutiontoinhibitE.coliO157:H7onfreshproduce.
1.Introduction
Consumers are now paying closer attention to the use
of arti?cial chemical preservatives to c
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