Antimicrobial Activity of Polyalthia longifolia (Sonn.) Thw. var. Pendula Leaf Extracts Against 91 Clinically Important Pathogenic Microbial Strains英文文献资料.docVIP

Chinese Medicine, 2010, 1, 31-38 doi:10.4236/cm.2010.12006 Published Online September 2010 (http://www.SciRP.org/journal/cm) Antimicrobial Activity of Polyalthia longifolia (Sonn.) Thw. var. Pendula Leaf Extracts Against 91 Clinically Important Pathogenic Microbial Strains Sumitra Chanda*, Rathish Nair Phytochemical, Pharmacological and Microbiological Laboratory Department of Biosciences, Saurashtra University, Rajkot, Gujarat, India E-mail: svchanda@ Received May 21, 2010; revised July 21, 2010; accepted July 26, 2010 Abstract The methanol, acetone and 1,4-dioxan fractions of leaves of Polyalthia longifolia (Sonn.) Thw. were evalu- ated for antibacterial and antifungal activity. 91 clinically important strains were used for the study which were both clinical isolates as well as identified strains. Piperacillin and gentamicin were used as standards for antibacterial assay, while nystatin and flucanazole were used as standards for antifungal assay. The antibac- terial activity was more pronounced against gram positive bacterial and fungal strains. Poor activity was shown against gram negative bacterial strains studied. Keywords: Antibacterial, Antifungal, Polyalthia longifolia, Clinical Isolates, Organic Solvent Extracts 1. Introduction 2. Materials and Methods 2.1. Plant Material Due to the increasing development of drug resistance in human pathogens as well as the appearance of undesir- able effect on certain antimicrobial agents, there is a need to search for new agents. The world health organi- zation in 1997 suggested that effective locally available plants be used as substitutes for drugs. Research work on medicinal plants be intensified and information on these plants be exchanged. This thought will go a long way in the scientific exploration of medicinal plants for the benefit of man and is likely to decrease the dependence on importance of drugs [1]. Polyalthia longifolia (An- nonaceae) i