Application of Flow Focusing to the Break-Up of a Magnetite Suspension Jet for the Production of Paramagnetic Microparticles英文文献资料.docVIP

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Application of Flow Focusing to the Break-Up of a Magnetite Suspension Jet for the Production of Paramagnetic Microparticles英文文献资料.doc

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Application of Flow Focusing to the Break-Up of a Magnetite Suspension Jet for the Production of Paramagnetic Microparticles英文文献资料

HindawiPublishingCorporation JournalofNanomaterials Volume2011,ArticleID527437,10pages doi:10.1155/2011/527437 ResearchArticle ApplicationofFlowFocusingtotheBreak-UpofaMagnetite SuspensionJetfortheProductionofParamagneticMicroparticles Luc′?aMart′?n-Banderas,1,2Rom′anGonz′alez-Prieto, 3 AlfonsoRodr′?guez-Gil, 4 MercedesFerna′ndez-Ar′evalo, 1 Mar′?aFlores-Mosquera, 2 Sebastia′nCh′avez, 4 andAlfonsoM. Ga?na′n-Calvo 2,5 1 2 3 4 5 DepartmentofPharmacyandPharmaceuticalTechnology,FacultyofPharmacy,UniversityofSeville,41012Seville,Spain RDDepartment,IngeniatricsTecnolog′?asS.L.,41900Camas(Seville),Spain CentroAndaluzdeBiolog′?aMolecularyMedicinaRegenerativa,CABIMER-CSIC,41092Seville,Spain DepartmentofGenetics,FacultyofBiology,UniversityofSeville,41012Seville,Spain DepartmentofAerospaceEngineeringandFluidsMechanics,ESI,UniversityofSeville,41092Seville,Spain CorrespondenceshouldbeaddressedtoAlfonsoM.Ga?n′an-Calvo,amgc@us.es Received31May2010;Accepted25July2010 AcademicEditor:LuSun Copyright?2011Luc′?aMart′?n-Banderasetal.ThisisanopenaccessarticledistributedundertheCreativeCommonsAttribution License,whichpermitsunrestricteduse,distribution,andreproductioninanymedium,providedtheoriginalworkisproperly cited. Paramagneticparticleso?eranextensiveimprovementinthemagneticseparationorpuri?cationofawidevarietyofprotein molecules. Most commercial paramagnetic particles are synthesized by laborious and costly procedures. A straightforward production of paramagnetic microparticles with homogeneous and selectable sizes using ?ow focusing (FF) technology is described in this work. The development of an initial formulation of a stable iron oxide suspension compatible with the FF requirementsisalsoreported.Theobtainedparticles,below10micronsindiameterandpresentingsmoothandreactivesurface, were codi?ed with an organic ?uorophore and showed excellent properties for covalent attachment of biomolecules such as proteinsanditssubsequentrecognitionby?owcytometry.Furthermore,particleswithsuitable