Application of RFLP-PCR-Based Identification for Sand Fly Surveillance in an Area Endemic for Kala-Azar in Mymensingh, Bangladesh英文文献资料.docVIP

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Application of RFLP-PCR-Based Identification for Sand Fly Surveillance in an Area Endemic for Kala-Azar in Mymensingh, Bangladesh英文文献资料.doc

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Application of RFLP-PCR-Based Identification for Sand Fly Surveillance in an Area Endemic for Kala-Azar in Mymensingh, Bangladesh英文文献资料

HindawiPublishingCorporation JournalofParasitologyResearch Volume2012,ArticleID467821,4pages doi:10.1155/2012/467821 ResearchArticle ApplicationofRFLP-PCR-BasedIdenti?cationfor SandFlySurveillanceinanAreaEndemicforKala-Azarin Mymensingh,Bangladesh MohammadSha?ulAlam, HirotomoKato, MizuhoFukushige, 1 2 3 YukikoWagatsuma, andMakotoItoh 4 3 1 2 3 4 ParasitologyLaboratory,InternationalCenterforDiarrhoealDiseaseResearch,Bangladesh(ICDDR,B), 68ShaheedTajuddinAhmedSarani,Mohakhali,Dhaka1212,Bangladesh LaboratoryofParasitology,DepartmentofDiseaseControl,GraduateSchoolofVeterinaryMedicine,HokkaidoUniversity, Sapporo060-0818,Japan DepartmentofEpidemiology,GraduateSchoolofComprehensiveHumanSciences,UniversityofTsukuba,Ibaraki, Tsukuba305-8577,Japan DepartmentofParasitology,AichiMedicalUniversitySchoolofMedicine,Nagakute480-1195,Japan CorrespondenceshouldbeaddressedtoMakotoItoh,macitoh@aichi-med-u.ac.jp Received14March2012;Accepted9April2012 AcademicEditor:AlvinA.Gajadhar Copyright?2012MohammadSha?ulAlametal. This is an open access article distributed under the Creative Commons AttributionLicense,whichpermitsunrestricteduse,distribution,andreproductioninanymedium,providedtheoriginalworkis properlycited. Mymensinghisthemostendemicdistrictforkala-azarinBangladesh.Phlebotomusargentipesremainstheonlyknownvector although a number of sand ?y species are prevalent in this area. Genotyping of sand ?ies distributed in a VL endemic area wasdevelopedbyaPCRandrestriction-fragment-lengthpolymorphism(RFLP)of18SrRNAgeneofsand?yspecies.Using theRFLP-PCRanalysiswith AfaIandHinfIrestrictionenzymes,P.argentipes,P.papatasi,andSergentomyiaspeciescouldbe identi?ed.Among1,055femalesand?iessuccessfullyanalyzedforthespeciesidenti?cationindividually,64.4%?ieswasclassi?ed as Sergentomyia species, whereas 35.6% was identi?ed as P. argentipes and no P. papatasi was found. Although infection of Leishmaniawithinthesand?ieswasindividuallyexaminedtargetingleishmanialm