Applications of Fluorescence in Situ Hybridization (FISH) for Detecting Genetic Changes in Hematological Malignancies英文文献资料.docVIP

Journal of Cancer Therapy, 2011, 2, 125-134 125 doi:10.4236/jct.2011.22015 Published Online June 2011 (http://www.SciRP.org/journal/jct) Applications of Fluorescence in Situ Hybridization (FISH) for Detecting Genetic Changes in Hematological Malignancies Deniz Ta?temir , Osman Demirhan , Emel Gürkan , Erdal Tun? , Nihal ?nand?kl?o?lu 1 2* 3 2 2 1 Vocational School of Health Services, Adiyaman University, Adiyaman, Turkey; 2 Department of Medical Biology and Genetics, ?ukurova University, Balcali, Adana, Turkey; Email: osdemir@.tr 3 Department of Hematology, ?ukurova University, Balcali, Adana, Turkey. Received March 31 , 2011; revised May 8 , 2011; accepted May 16 , 2011. st th th ABSTRACT Fluorescence in situ hybridization (FISH) has become an important tool both for defining initial chromosomal abnormalities within a disease process, and for monitoring response to therapy as well as minimal residual disease. We report the results of interphase FISH (iFISH) analysis of 92 patients. We have used five different FISH probes to detect common cytogenetic rearrangements associated with hematological malignancies. A total of 83 patients were screened for BCR/ABL gene rearrangements. Displayed iFISH patterns of BCR/ABL gene rearrangements in 37.3% of patients (31/83) ranged between 10% to 98%. In addition, while 3 patients and one patient with AML showed t(15; 17) (12.5%) and inv(16; 16) (8.3%) respectively, t(8; 21) was not found. Furthermore, secondary chromosomal aberrations (6.5% of all cases) were clearly non random in the present study. The diagnosis of BCR/ABL gene rearrangements are likely become an important tool for the monitoring of thera