ARR5 and ARR6 Mediate Tissue Specific Cross-talk between Auxin and Cytokinin in Arabidopsis英文文献资料.docVIP

American Journal of Plant Sciences, 2011, 2, 549-553 549 doi:10.4236/ajps.2011.24065 Published Online October 2011 (http://www.SciRP.org/journal/ajps) ARR5 and ARR6 Mediate Tissue Specific Cross-talk between Auxin and Cytokinin in Arabidopsis Aparna Kakani, Zhaohua Peng Department of Biochemistry and Molecular Biology, Mississippi State University, Starkville, USA. Email: zp7@ Received July 23 , 2011; revised September 1 , 2011; accepted September 10 , 2011. rd st th ABSTRACT Auxin and cytokinin interaction plays an essential role in a wide range of plant growth and developmental processes. The interaction consequence of the two hormones is highly tissue specific. The molecular mechanisms underlying the tissue specificity are largely unknown. Here we show that the cytokinin signaling pathway key components ARR5 and ARR6 respond to auxin with a highly tissue specific and contrasted pattern in Arabidopsis seedlings and calli in the presence of cytokinin. Our results suggest that the two highly homologous but functionally distinct genes, ARR5 and ARR6, play a critical role in mediating tissue specific interaction between auxin and cytokinin. Keywords: ARR5, ARR6, Auxin, Cytokinin, Callus, Arabidopsis, Auxin and Cytokinin Interaction 1. Introduction kinin overproducing lines of Nicotiana glutinosa trans- formed with the bacterial cytokinin biosynthesis gene ipt (isopentenyl transferase) and in maize and pea treated with exogenously applied cytokinin [10-12], probably via cytokinin inhibition of enzymes that conjugate free IAA [13]. Meanwhile, it has also been reported that cytokinin overexpression lead to down regulation of the IAA pool in tobacco [14]. The discrepancy among different studies suggests that further investigations are required for a complete picture of th