Assessment of a short phylogenetic marker based on comparisons of 3’ end 16S rDNA and 5’ end 16S-23S ITS nucleotide sequences of the Bacillus cereus group英文文献资料.docVIP

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Assessment of a short phylogenetic marker based on comparisons of 3’ end 16S rDNA and 5’ end 16S-23S ITS nucleotide sequences of the Bacillus cereus group英文文献资料.doc

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Assessment of a short phylogenetic marker based on comparisons of 3’ end 16S rDNA and 5’ end 16S-23S ITS nucleotide sequences of the Bacillus cereus group英文文献资料

Vol.2, No.10, 1113-1118 (2010) Natural Science /10.4236/ns.2010.210138 Assessment of a short phylogenetic marker based on comparisons of 3’ end 16S rDNA and 5’ end 16S-23S ITS nucleotide sequences of the Bacillus cereus group Sabarimatou Yakoubou , Jean-Charles C?té * 1,2 1 1 Agriculture and Agri-Food Canada, Research Centre, Gouin Blvd, St-Jean-sur-Richelieu, Canada; *Corresponding Author: Jean-Charles.Cote@agr.gc.ca; Département des Sciences Biologiques, Université du Québec à Montréal, Succ.“Centre-Ville” Montréal, Canada. 2 Received 28 May 2010; revised 30 June 2010; accepted 5 July 2010. ABSTRACT Keywords: Bacillus cereus; 16S rDNA; 16S-23S ITS; Phylogeny A short phylogenetic marker previously used in the reconstruction of the Order Bacillales and the genus Bacillus was assessed here at a lower taxa level: species in the Bacillus cereus group: B. anthracis, B. cereus, B. thuringiensis and B. weihenstephanensis. This maker is 220 bp in length. It is a combination of 150 bp at the 3’ end of the 16S rDNA and 70 bp at the 5’ end of the 16S-23S ITS sequence. Three additional Bacillus species, B. halodurans, B. licheniformis and B. subtilis, and Clostridium tetani were included for comparison purposes. A total of eight bacte- rial species and 12 strains were analyzed. A boot- strapped neighbor-joining tree was inferred from comparative analyses of all allelic sequences of the bacterial species and strains under study. Based on its topology, four major Groups were revealed at the 90% nucleotide sequence identi- ties, Group I to IV. Group I contains all alleles of the Bacillus cereus group. Group II contains all alleles of B. halodurans. Group III contains all alleles of B. licheniformis and B. subtilis. Group IV contains all alleles of Clostridium tetani. The 220 bp phylogenetic marker used here could resolve different species from different genera. At the gen