Biochemical Characterization of Bovine Brain Myristoyl-CoAProtein N-Myristoyltransferase Type 2英文文献资料.docVIP

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Biochemical Characterization of Bovine Brain Myristoyl-CoAProtein N-Myristoyltransferase Type 2英文文献资料.doc

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Biochemical Characterization of Bovine Brain Myristoyl-CoAProtein N-Myristoyltransferase Type 2英文文献资料

HindawiPublishingCorporation JournalofBiomedicineandBiotechnology Volume2009,ArticleID907614,9pages doi:10.1155/2009/907614 ResearchArticle BiochemicalCharacterizationofBovineBrain Myristoyl-CoA:ProteinN-MyristoyltransferaseType2 PonniahSelvakumar,AshakumaryLakshmikuttyamma,andRajendraK.Sharma DepartmentofPathologyandLaboratoryMedicine,CollegeofMedicineandHealthResearchDivision,SaskatchewanCancerAgency, UniversityofSaskatchewan,20CampusDrive,Saskatoon,SK,CanadaS7N4H4 CorrespondenceshouldbeaddressedtoRajendraK.Sharma,rajendra.sharma@saskcancer.ca Received6February2009;Revised9June2009;Accepted15June2009 RecommendedbyMatthewB.Wheeler ProteinN-myristoylationisalipidicmodi?cationwhichreferstothecovalentattachmentofmyristate,a14-carbonsaturatedfatty acid,totheN-terminalglycineresidueofanumberofmammalian,viral,andfungalproteins.Inthispaper,wehaveclonedthe genecodingformyristoyl-CoA:proteinN-myristoyltransferase(NMT)fromBostarusbrain.Theopenreadingframecodesfor a410-amino-acidproteinandoverexpressedinEscherichiacoli.KineticstudiessuggestedthatbovinebrainNMT2andhuman NMT1showsigni?cantdi?erencesintheirpeptidesubstratespeci?cities.ThemetalionCa hadstimulatorye?ectsonNMT2 2+ activitywhileMn andZn inhibitedtheenzymeactivity.Inaddition,NMT2activitywasinhibitedbyvariousorganicsolvents 2+ 2+ andotherdetergentswhileNMT1hadastimulatorye?ect.BiochemicalcharacterizationsuggestedthatbothformsofNMThave uniquecharacteristics.FurtheranalysistowardsfunctionalroleNMT2willleadthedevelopmentoftherapeutictargetforthe progressionofvariousdiseasessuchascancer,cardiovasculardiseases,andneurodegenerativediseases. Copyright?2009PonniahSelvakumaretal.ThisisanopenaccessarticledistributedundertheCreativeCommonsAttribution License,whichpermitsunrestricteduse,distribution,andreproductioninanymedium,providedtheoriginalworkisproperly cited. 1.Introduction ferase(NMT)whichisamemberoftheGNATsuperfamily of proteins [1–8]. Despite recent advances in the protein myristoylation?eld,thespeci?citiesof