Background Estimation of Biomedical Raman Spectra Using a Geometric Approach英文文献资料.docVIP

Hindawi Publishing Corporation Spectroscopy: An International Journal Volume 27 (2012), Issue 5-6, Pages 311–317 doi:10.1155/2012/530791 Background Estimation of Biomedical Raman Spectra Using a Geometric Approach Nikolaos Kourkoumelis, Alexandros Polymeros, and Margaret Tzaphlidou 1 2 1 1Department of Medical Physics, Medical School, University of Ioannina, Ioannina 45110, Greece 2Department of Physics, University of Ioannina, Ioannina 45110, Greece Correspondence should be addressed to Nikolaos Kourkoumelis, nkourkou@cc.uoi.gr Copyright ? 2012 Nikolaos Kourkoumelis et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract. Raman spectroscopy grows into an essential tool for biomedical applications. Nevertheless, the weak Raman signal associated mainly with biological samples is often obscured by a broad background signal due to the intrinsic ?uorescence of the organic molecules present, making further analysis unfeasible. A computational geometry method based on the de?nition of convex hull is described to estimate the background from Raman spectra of samples with biological interest. The method is semiautomated requiring sample-dependent user intervention. It does not depend, however, on curve ?tting, requires no information about background distribution or source, and keeps the original spectral data intact. Keywords: Raman spectroscopy, background estimation, computational geometry 1. Introduction Raman spectroscopy has been extensively applied in recent years in a variety of biological research ran- ging from the in situ tissue diagnosis to the analysis of subcellular components. Being a vibrational spectroscopic technique based on inelastic scattering, Raman spectrosco