Biocompatibility of Porous Spherical Calcium Carbonate Microparticles on Hela Cells英文文献资料.docVIP

World Journal of Nano Science and Engineering, 2012, 2, 25-31 25 /10.4236/wjnse.2012.21005 Published Online March 2012 (http://www.SciRP.org/journal/wjnse) Biocompatibility of Porous Spherical Calcium Carbonate Microparticles on Hela Cells Yaran Zhang , Ping Ma , Yao Wang , Juan Du , Qi Zhou , Zhihong Zhu , Xu Yang , Junlin Yuan 1 2 3 1 1 4 1* 1* 1 Laboratory of Environmental Sciences, College of Life Sciences, Central China Normal University, Wuhan, China Basic Medical College, Xianning University, Xianning, China School of Life Sciences, Fudan University, Shanghai, China Nanotechnology Institute, College of Physical Science and Technology, Central China Normal University, Wuhan, China 2 3 4 Email: yangxu@, yuanjl@ * * Received November 30, 2011; revised December 12, 2011; accepted January 2, 2012 ABSTRACT Recently there has been a wide concern on inorganic nanoparticles as drug delivery carriers. CaCO3 particles have shown promising potential for the development of carriers for drugs, but little research had been performed regarding their safe dosage for maximizing the therapeutic activity without harming biosystems. In this study, we assessed the biological safety of porous spherical CaCO3 microparticles on Hela cells. The reactive oxygen species (ROS), glu- tathione (GSH), carbonyl content in proteins (CCP), DNA-protein crosslinks (DPC) and cell viability were measured. Results showed that with the exposure concentration increase, ROS and CCP in Hela cells presented a significant in- crease but GSH contents in Hela cells and cell viability showed a significant decrease respectively compared with the control. DPC coefficient ascended, but no st