Bioconversion of Agricultural Waste to Ethanol by SSF Using Recombinant Cellulase from Clostridium thermocellum英文文献资料.docVIP

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Bioconversion of Agricultural Waste to Ethanol by SSF Using Recombinant Cellulase from Clostridium thermocellum英文文献资料.doc

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Bioconversion of Agricultural Waste to Ethanol by SSF Using Recombinant Cellulase from Clostridium thermocellum英文文献资料

SAGE-HindawiAccesstoResearch EnzymeResearch Volume2011,ArticleID340279,6pages doi:10.4061/2011/340279 ResearchArticle BioconversionofAgriculturalWastetoEthanolbySSFUsing RecombinantCellulasefromClostridiumthermocellum RuchiMutreja, DebasishDas, DineshGoyal, andArunGoyal 1 1 2 1 1 2 DepartmentofBiotechnology,IndianInstituteofTechnologyGuwahati,Guwahati781039,Assam,India DepartmentofBiotechnologyandEnvironmentalSciences,ThaperUniversity,BhadsonRoad,Patiala140007,Punjab,India CorrespondenceshouldbeaddressedtoDebasishDas,debasishd@iitg.ernet.inandArunGoyal,arungoyl@iitg.ernet.in Received1March2011;Revised20May2011;Accepted21May2011 AcademicEditor:AlaneBeatrizVermelho Copyright?2011RuchiMutrejaetal.ThisisanopenaccessarticledistributedundertheCreativeCommonsAttributionLicense, whichpermitsunrestricteduse,distribution,andreproductioninanymedium,providedtheoriginalworkisproperlycited. Thee?ectofdi?erentpretreatmentmethods,temperature,andenzymeconcentrationonethanolproductionfrom8lignocellu- losicagrowastebysimultaneoussacchari?cationandfermentation(SSF)usingrecombinantcellulaseandSaccharomycescerevisiae werestudied.RecombinantcellulasewasisolatedfromE.coliBL21cellstransformedwithCtLic26A-Cel5-CBM11full-lengthgene fromClostridiumthermocellumandproducedinbothbatchandfed-batchprocesses.ThemaximumcellODandspeci?cactivity inbatchmodewere1.6and1.91U/mg, respectively, whereas inthefed-batchmode,maximumcellODandspeci?cactivity were3.8and3.5U/mg,respectively,displayinga2-foldincrease.Eightsubstrates,Syzygiumcumini(jamun),Azadirachtaindica (neem),Saracensindica(asoka),bambusadendrocalmus(bamboo),Populasnigra(poplar),Achnatherumhymenoides(wildgrass), Eucalyptusmarginata(eucalyptus),andMangiferaindica(mango),weresubjectedtoSSF.Ofthreepretreatments,acid,alkali,and steamexplosion,acidpretreatmentSyzygiumcumini(Jamun)at30 Cgavemaximumethanolyieldof1.42g/L. ? 1.Introduction of sugars that can be converted to ethanol. The potential forus