Biodegradation of Aged Residues of Atrazine and Alachlor in a Mix-Load Site Soil by Fungal Enzymes英文文献资料.docVIP

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Biodegradation of Aged Residues of Atrazine and Alachlor in a Mix-Load Site Soil by Fungal Enzymes英文文献资料.doc

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Biodegradation of Aged Residues of Atrazine and Alachlor in a Mix-Load Site Soil by Fungal Enzymes英文文献资料

HindawiPublishingCorporation AppliedandEnvironmentalSoilScience Volume2011,ArticleID658569,10pages doi:10.1155/2011/658569 ResearchArticle BiodegradationofAgedResiduesofAtrazineandAlachlorin aMix-LoadSiteSoilbyFungalEnzymes AnastasiaE.M.Chirnside, WilliamF.Ritter, andMarkRadosevich 1 1 2 1 2 BioresourcesEngineering,UniversityofDelaware,TownsendHall,Rm264,531S.CollegeAve,Newark,DE19716-2140,USA BiosystemsEngineeringandSoilScience,UniversityofTennessee,2506E.J.ChapmanDr.,Knoxville,TN37996-4531,USA CorrespondenceshouldbeaddressedtoAnastasiaE.M.Chirnside,aemc@ Received14April2011;Revised22July2011;Accepted24July2011 AcademicEditor:TeodoroMiano Copyright?2011AnastasiaE.M.Chirnsideetal. This is an open access article distributed under the Creative Commons AttributionLicense,whichpermitsunrestricteduse,distribution,andreproductioninanymedium,providedtheoriginalworkis properlycited. Soils from bulk pesticide mixing and loading (mix-load) sites are often contaminated with a complex mixture of pesticides, herbicides,andotherorganiccompoundsusedinpesticideformulationsthatlimitsthesuccessofremediatione?orts.Therefore, there is a need to ?nd remediation strategies that can successfully clean up these mix-load site soils. This paper examined thedegradationofatrazine(2-chloro-4-ethylamino-6-isopropylamino-S-triazine;AT)andalachlor(2-chloro-2,6-diethyl-N- [methoxymethyl]-acetanilide)incontaminatedmix-loadsitesoilutilizinganextracellularfungalenzymesolutionderivedfrom thewhiterotfungus,Phanerochaetechrysosporium,growninapackedbedbioreactor.Thirty-twopercentofATand54%ofALwere transformedinthebiometers.Thepseudo?rst-orderrateconstantforATandALbiodegradationwas0.0882d?1and0.2504d?1 , respectively.Thehalf-life(t1/2)forATandALwas8.0and3.0days,respectively.ComparedtoAT,theinitialdisappearanceofAL proceededatafasterrateandresultedinagreateramountofALtransformed.BasedonthenetCo2evolvedfromthebiometers, about4%oftheATandALinitiallypresentin