Biosynthesis and Immobilization of Biofunctional Allophycocyanin英文文献资料.docVIP

HindawiPublishingCorporation Evidence-BasedComplementaryandAlternativeMedicine Volume2011,ArticleID751452,6pages doi:10.1155/2011/751452 ResearchArticle BiosynthesisandImmobilizationof BiofunctionalAllophycocyanin YingjieChen,1,2ShaofangLiu,1,2YulinCui,1,2PengJiang, 1 HuaxinChen, FuchaoLi, andSongQin 1 1 1 1 2 KeyLaboratoryofExperimentalMarineBiology,InstituteofOceanology,ChineseAcademyofSciences,Qingdao266071,China GraduateUniversityofChineseAcademyofSciences,Beijing100049,China CorrespondenceshouldbeaddressedtoPengJiang,jiangpeng@andSongQin,sqin@ Received14January2011;Revised27April2011;Accepted20June2011 Copyright?2011YingjieChenetal.ThisisanopenaccessarticledistributedundertheCreativeCommonsAttributionLicense, whichpermitsunrestricteduse,distribution,andreproductioninanymedium,providedtheoriginalworkisproperlycited. The holo-allophycocyanin-α subunit, which has various reported pharmacological uses, was biosynthesized with both Strep- II-tag and His-tag at the N-terminal in Escherichia coli. The streptavidin-binding ability resulting from the Strep II-tag was con?rmedbyWesternblot.Additionally,themetal-chelatingabilityderivingfromtheHis-tagnotonlyfacilitateditspuri?cationby immobilizedmetal-iona?nitychromatographybutalsopromoteditsimmobilizationonZn(II)-decoratedsilica-coatedmagnetic nanoparticles. The holo-allophycocyanin-α subunit with streptavidin-binding ability was thereby immobilized on magnetic nanoparticles.Magneticnanoparticlesarepromisingasdrugdeliveryvehiclesfortargetingandlocatingattumors.Thus,basedon geneticengineeringandnanotechnology,weprovideapotentialstrategytofacilitatethebiomodi?cationandtargeteddeliveryof pharmacologicalproteins. 1.Introduction attentionwithrespecttothepossibilitythatitmightavoid chemical manipulation for the conjugation of antibodies tobioactiveproteins[14–16].Inaddition,ashortHis-tag, whichisthemostcommonlyusedtag,canbeeasilyfused to proteins without impairing their function and confer metalchelati