Biotechnological Potential of Agro Residues for Economical Production of Thermoalkali-Stable Pectinase by Bacillus pumilus dcsr1 by Solid-State Fermentation and Its Efficacy in the Treatment of Ramie Fibres英文文献资料.docVIP

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Biotechnological Potential of Agro Residues for Economical Production of Thermoalkali-Stable Pectinase by Bacillus pumilus dcsr1 by Solid-State Fermentation and Its Efficacy in the Treatment of Ramie Fibres英文文献资料.doc

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Biotechnological Potential of Agro Residues for Economical Production of Thermoalkali-Stable Pectinase by Bacillus pumilus dcsr1 by Solid-State Fermentation and Its Efficacy in the Treatment of Ramie Fibres英文文献资料

HindawiPublishingCorporation EnzymeResearch Volume2012,ArticleID281384,7pages doi:10.1155/2012/281384 ResearchArticle BiotechnologicalPotentialofAgroResiduesforEconomical ProductionofThermoalkali-StablePectinasebyBacilluspumilus dcsr1bySolid-StateFermentationandItsEf?cacyin theTreatmentofRamieFibres DeepakChandSharma andT.Satyanarayana 1 2 1 2 DepartmentofMicrobiology,ChaudharyCharanSinghUniversity,Meerut250004,India DepartmentofMicrobiology,UniversityofDelhiSouthCampus,BenitoJuarezRoad,NewDelhi110021,India CorrespondenceshouldbeaddressedtoT.Satyanarayana,tsnarayana@ Received16April2012;Revised26June2012;Accepted26June2012 AcademicEditor:JoseM.Guisan Copyright?2012D.C.SharmaandT.Satyanarayana. This is an open access article distributed under the Creative Commons AttributionLicense,whichpermitsunrestricteduse,distribution,andreproductioninanymedium,providedtheoriginalworkis properlycited. TheproductionofathermostableandhighlyalkalinepectinasebyBacilluspumilusdcsr1wasoptimizedinsolid-statefermentation (SSF) and the impact of various treatments (chemical, enzymatic, and in combination) on the quality of ramie ?bres was investigated.Maximumenzymetiter(348.0± 11.8Ug?1 DBB)inSSFwasattained,whenamixtureofagro-residues(sesame oilseedcake,wheatbran,andcitruspectin,1:1:0.01)wasmoistenedwithmineralsaltsolution(aw0.92,pH9.0)atasubstrate- to-moisteningagentratioof1:2.5andinoculatedwith25%of24holdinoculum,in144hat40 C.Parametricoptimization ? inSSFresultedin1.7-foldenhancementintheenzymeproductionascomparedtothatrecordedinunoptimizedconditions.A 14.2-foldhigherenzymeproductionwasattainedinSSFascomparedtothatinsubmergedfermentation(SmF).Thetreatment withtheenzymesigni?cantlyimprovedtensilestrengthandYoung’smodulus,reductioninbrittleness,rednessandyellowness, andincreaseinthestrengthandbrightnessoframie?bres. 1.Introduction offruitjuicesandwines[17,18],whilealkalinepectinases ?ndapplicationsintextileindustryforrettingofplant?bres, manufactur