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Calibration of Surface Plasmon Resonance Imager for Biochemical Detection英文文献资料
HindawiPublishingCorporation
InternationalJournalofElectrochemistry
Volume2012,ArticleID421692,5pages
doi:10.1155/2012/421692
ResearchArticle
CalibrationofSurfacePlasmonResonanceImagerfor
BiochemicalDetection
T.Ktari,H.Baccar,M.B.Mejri,andA.Abdelghani
NanotechnologyLaboratory,INSAT,CarthageUniversity,CentreUrbainNord,1080CharguiaCedex,Tunisia
CorrespondenceshouldbeaddressedtoA.Abdelghani,aabdelghan@yahoo.fr
Received13July2011;Revised28August2011;Accepted4September2011
AcademicEditor:UlkuAnik
Copyright?2012T.Ktarietal.ThisisanopenaccessarticledistributedundertheCreativeCommonsAttributionLicense,which
permitsunrestricteduse,distribution,andreproductioninanymedium,providedtheoriginalworkisproperlycited.
WepresentanewSurfacePlasmonResonanceimager(SPRi)basedonimmobilizedT4-phageforbacteriadetection.First,we
presentthesensitivityoftheSPRimagertowardsrefractiveindexvariationforbiosensorapplication.TheSPRimagercanbe
calibratedversusdi?erentpercentageoftriethyleneglycolmixtureinultrapurewater.Thesystemcanbeusedasarefractometer
withsensitivitybelow5×10?5intherangeof1.33300–1.34360.Second,bacteriophage(T4-phage)canbephysisorbedongold
microarrayspotsforbacteriadetection.Thekineticphysisorptionofdi?erentconcentrationsofT4-phagescanbeobservedinreal
time.Finally,twotypesofbacteriasuchasE.coli(gramnegative)andLactobacillus(grampositive)wereusedforpositiveand
negativetests.TheresultsshowaselectivityofT4-phagetowardE.coliwithadetectionlimitbelow10 CFU/mLandwithgood
4
reproducibility.
1.Introduction
(SPRi), the whole chip is literally visualized using a CCD
videocamera[15].Simultaneously,theequipmentmonitors
thechangesinlightre?ectivityoverthewholesurfaceata
?xed resonance angle and in real time. The local changes
atthesurfaceofthechiprecordedaschangesofrefractive
index provide detailed information on any interaction of
kineticprocesstakingplace.Thismakespossiblemultiplexed
label-freedetectionusingsensorsinanarrayformat,greatly
improving experimental throughput and robustnes
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