Calibration of Surface Plasmon Resonance Imager for Biochemical Detection英文文献资料.docVIP

Calibration of Surface Plasmon Resonance Imager for Biochemical Detection英文文献资料.doc

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Calibration of Surface Plasmon Resonance Imager for Biochemical Detection英文文献资料

HindawiPublishingCorporation InternationalJournalofElectrochemistry Volume2012,ArticleID421692,5pages doi:10.1155/2012/421692 ResearchArticle CalibrationofSurfacePlasmonResonanceImagerfor BiochemicalDetection T.Ktari,H.Baccar,M.B.Mejri,andA.Abdelghani NanotechnologyLaboratory,INSAT,CarthageUniversity,CentreUrbainNord,1080CharguiaCedex,Tunisia CorrespondenceshouldbeaddressedtoA.Abdelghani,aabdelghan@yahoo.fr Received13July2011;Revised28August2011;Accepted4September2011 AcademicEditor:UlkuAnik Copyright?2012T.Ktarietal.ThisisanopenaccessarticledistributedundertheCreativeCommonsAttributionLicense,which permitsunrestricteduse,distribution,andreproductioninanymedium,providedtheoriginalworkisproperlycited. WepresentanewSurfacePlasmonResonanceimager(SPRi)basedonimmobilizedT4-phageforbacteriadetection.First,we presentthesensitivityoftheSPRimagertowardsrefractiveindexvariationforbiosensorapplication.TheSPRimagercanbe calibratedversusdi?erentpercentageoftriethyleneglycolmixtureinultrapurewater.Thesystemcanbeusedasarefractometer withsensitivitybelow5×10?5intherangeof1.33300–1.34360.Second,bacteriophage(T4-phage)canbephysisorbedongold microarrayspotsforbacteriadetection.Thekineticphysisorptionofdi?erentconcentrationsofT4-phagescanbeobservedinreal time.Finally,twotypesofbacteriasuchasE.coli(gramnegative)andLactobacillus(grampositive)wereusedforpositiveand negativetests.TheresultsshowaselectivityofT4-phagetowardE.coliwithadetectionlimitbelow10 CFU/mLandwithgood 4 reproducibility. 1.Introduction (SPRi), the whole chip is literally visualized using a CCD videocamera[15].Simultaneously,theequipmentmonitors thechangesinlightre?ectivityoverthewholesurfaceata ?xed resonance angle and in real time. The local changes atthesurfaceofthechiprecordedaschangesofrefractive index provide detailed information on any interaction of kineticprocesstakingplace.Thismakespossiblemultiplexed label-freedetectionusingsensorsinanarrayformat,greatly improving experimental throughput and robustnes

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