cAMP Activates The generation of Reactive Oxygen Species and Inhibits The Secretion of IL-6 in Peripheral Blood Mononuclear Cells from Type 2 Diabetic Patients英文文献资料.docVIP

Resea RCh pa peR Resea RCh pa peR Oxidative Medicine and Cellular Longevity 2:5, 317-321; November/December 2009; ? 2009 Landes Bioscience ca Mp activates the generation of reactive oxygen species and inhibits the secretion of IL-6 in peripheral blood mononuclear cells from type 2 diabetic patients Camila a rmond Isoni, 1 érica a breu Borges, Clara a raújo Veloso, Rafael Teixeira Mattos, Miriam Martins Chaves 1 1 1 2 and José a ugusto Nogueira-Machado * 1, 1 Núcleo de Pós-Gradua??o e Pesquisa; Hospital Santa Casa de Belo Horizonte; Belo Horizonte; Minas Gerais, Brasil; Departamento de Bioquímica e Imunologia; 2 Universidade Federal de Minas Gerais; Belo Horizonte; Minas Gerais, Brazil Key words: ROS, IL-6, cAMP, diabetes, peripheral blood mononuclear cells peripheral blood mononuclear cells (pBMNC) from patients withtype 2 diabetes (DM2) have generated higher levels of reactive oxygen species (ROs) that were higher than those in cells from healthy individuals. In the presence of a ca Mp- elevating agent, ROS production was signi?cantly activated in PBMNC from DM2 patients but it was inhibited in cells from healthy subjects. higher levels of IL-6 has been detected in the supernatant of pBMNC cultures from DM2 patients in comparison withhealthy controls. When cells were cultured in the presence of a ca Mp-elevating agent, the level of IL-6 decreased has by 46% in the supernatant of pBMNC from DM2 patients but it remained unaltered in controls. No cor- relations between ROs and IL-6 levels in pBMNC from DM2 patients or controls have been observed. secretions of IL-4 or IFN by pBMNC from patients or controls have not been affected by the elevation of ca Mp. ca Mp elevating agents have activated the production of harmful reactive oxidant down modulated IL-6