Casein Kinase Iγ2 Impairs Fibroblasts Actin Stress Fibers Formation and Delays Cell Cycle Progression in G1英文文献资料.docVIP

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Casein Kinase Iγ2 Impairs Fibroblasts Actin Stress Fibers Formation and Delays Cell Cycle Progression in G1英文文献资料.doc

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Casein Kinase Iγ2 Impairs Fibroblasts Actin Stress Fibers Formation and Delays Cell Cycle Progression in G1英文文献资料

HindawiPublishingCorporation InternationalJournalofCellBiology Volume2012,ArticleID684684,15pages doi:10.1155/2012/684684 ResearchArticle CaseinKinaseIγ2ImpairsFibroblastsActinStressFibers FormationandDelaysCellCycleProgressioninG1 MathieuLatreille,AfnanAbu-Thuraia,RossellaOliva,DongmeiZuo,andLouiseLarose PolypeptideLaboratory,DepartmentofMedicine,McGillUniversity,Montreal,QC,CanadaH3A2B2 CorrespondenceshouldbeaddressedtoLouiseLarose,louise.larose@mcgill.ca Received6September2011;Revised1December2011;Accepted5December2011 AcademicEditor:LizaPon Copyright?2012MathieuLatreilleetal. This is an open access article distributed under the Creative Commons Attribution License,whichpermitsunrestricteduse,distribution,andreproductioninanymedium,providedtheoriginalworkisproperly cited. Actincytoskeletonremodelingisundertheregulationofmultipleproteinswithvariousactivities.Here,wedemonstratethatthe γ2isoformofCaseinKinaseI(CKIγ2)ispartofanovelmolecularpathregulatingtheformationofactinstress?bers.Weshowthat overexpressionofCKIγ2in?broblastsalterscellmorphologybyimpairingactinstress?bersformation.Wedemonstratethatthis isconcomitantwithincreasedphosphorylationoftheCDKinhibitorp27 onCKIγ2catalyticactivity.Moreover,wereportthatroscovitine,apotentinhibitorofcyclin-dependentkinases,includingCdk5, decreasesp27 proteinlevelsandrestoresactinstress?bersformationinCKIγ2overexpressingcells,suggestingtheexistenceofa CKIγ2-Cdk5-p27 -RhoApathwayinregulatingactinremodeling.Ontheotherhand,wealsoshowthatinamannerindependent Kip andlowerlevelsofactivatedRhoA,andisdependent Kip Kip ofitscatalyticactivity,CKIγ2delayscellcycleprogressionthroughG1.Collectivelyour?ndingsrevealthatCKIγ2isanovelplayer inthecontrolofactincytoskeletondynamicsandcellproliferation. 1.Introduction on its abundance and nuclear localization to inhibit the cyclin-dependentkinases(CDKs),inhibitsRhoAactivation The Rho family of GTPases comprising RhoA, Rac1, and Cdc42 regulates the organization of the cytoskeleton in eukaryot