CD9 Expression by Human Granulosa Cells and Platelets as a Predictor of Fertilization Success during IVF英文文献资料.docVIP

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CD9 Expression by Human Granulosa Cells and Platelets as a Predictor of Fertilization Success during IVF英文文献资料.doc

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CD9 Expression by Human Granulosa Cells and Platelets as a Predictor of Fertilization Success during IVF英文文献资料

HindawiPublishingCorporation ObstetricsandGynecologyInternational Volume2010,ArticleID192461,7pages doi:10.1155/2010/192461 ResearchArticle CD9ExpressionbyHumanGranulosaCellsandPlateletsas aPredictorofFertilizationSuccessduringIVF CarolynR.Jaslow, KyleS.Patterson, ShilaCholera, LisaK.Jennings, 1 2 3 3 RaymondW.Ke,2,4andWilliamH.Kutteh 2,4 1 2 3 DepartmentofBiology,RhodesCollege,2000NorthParkway,Memphis,TN38112,USA DepartmentofObstetricsandGynecology,UniversityofTennesseeHealthScienceCenter,Memphis,TN38152,USA DepartmentofInternalMedicine,TheVascularBiologyCenterofExcellence,UniversityofTennesseeHealthScienceCenter,Memphis, TN38152,USA 4 FertilityAssociatesofMemphis,80HumphreysCenter,Suite307,Memphis,TN38120,USA CorrespondenceshouldbeaddressedtoWilliamH.Kutteh,wkutteh@ Received27May2010;Revised16July2010;Accepted28July2010 AcademicEditor:EdwardV.Younglai Copyright?2010CarolynR.Jaslowetal. This is an open access article distributed under the Creative Commons Attribution License,whichpermitsunrestricteduse,distribution,andreproductioninanymedium,providedtheoriginalworkisproperly cited. Objective. To determine whether CD9 expression on human granulosa cells (GCs) and platelets could predict the success of conventional fertilization of human oocytes during in vitro fertilization (IVF). Methods. Thirty women undergoing IVF for nonmale factor infertility participated. Platelets from venous blood and GCs separated from retrieved oocytes were prepared forimmuno?uorescence.Flowcytometryquanti?edthepercentofGCsexpressingCD9,andCD9surfacedensityonGCsand platelets. Fertilization rate was determined for the total number of oocytes, and the number of mature oocytes per patient. Correlationstestedforsigni?cantrelationships(P .05)betweenfertilizationratesandCD9expression.Results.CD9surface densityonhumanGCsisinverselycorrelatedwithfertilizationrateofoocytes(P=.04),buttherelationshipwasweak.Conclusion. Morestudiesareneededtodet