Cellular Prion Protein and Sexual Dimorphic Areas in Rodents. Correlates with Alzheimer Disease英文文献资料.docVIP

Neuroscience Medicine, 2011, 2, 384-391 doi:10.4236/nm.2011.24051 Published Online December 2011 (http://www.SciRP.org/journal/nm) Cellular Prion Protein and Sexual Dimorphic Areas in Rodents. Correlates with Alzheimer Disease Mar Cuadrado-Tejedor , Ana Irujo , Beatriz Paternain , María Jesús Madrid , José Luis Velayos 1 1* 1 2,3 1,2 1 Department of Anatomy, Faculty of Medicine, University of Navarra, Pamplona, Spain; 2 IMMA (Institute of Applied Molecular Department of Basic Medical Sci- Medicine), Faculty of Medicine, University San Pablo CEU, Boadilla dl Monte, Madrid, Spain; ences, University San Pablo-CEU, Boadilla dl Monte, Madrid, Spain. * E-mail: airujo@unav.es 3 Received October 6 , 2011; revised November 20 , 2011; accepted November 29 , 2011. th th th ABSTRACT The cellular prion protein (PrPC) expression was analyzed by western-blot in the rat, in two different dimorphic brain areas such as the anterior hypothalamic and the preoptic areas. In both cases, the PrPC expression was increased in males, implying a sexual dimorphism for the PrPC protein. The study was also made in other two brain areas, frontal cortex and hyppocampus (a clearly dimorphic area); in this case, mice of different ages of both sexes were used. In both brain areas analyzed, although the PrPC expression was increased with age until the adult age (38 weeks), it was de- creased in aged animals (56 weeks) in both sexes. The PrPC expression in mouse hippocampus was predominant in males in comparison to females. Moreover, the non-glycosylated band was increased with age and this increase was parallel with the increase