Cellular Profiles in Peripheral Blood Accompanying Particular Asthmatic Response Types英文文献资料.docVIP

International Journal of Clinical Medicine, 2012, 3, 485-497 485 /10.4236/ijcm.2012.36088 Published Online November 2012 (http://www.SciRP.org/journal/ijcm) Cellular Profiles in Peripheral Blood Accompanying Particular Asthmatic Response Types Zdenek Pelikan Allergy Research Foundation, Breda, The Netherlands. Email: zpelikan@casema.nl Received July 23 , 2012; revised August 26 , 2012; accepted September 13 , 2012 rd th th ABSTRACT Background: Patients with allergic bronchial asthma develop various asthmatic response types to bronchial challenge with allergen, such as immediate (IAR), late (LAR), dual late (DLAR) or delayed (DYAR), displaying different clinical, immunologic and pharmacologic features. This study deals with count changes of particular blood cells accompanying the IAR, LAR and DYAR. Methods: In 63 patients developing 22 IAR, 26 LAR and 15 DYAR, the repeated allergen challenges were supplemented with recording of blood cell counts, Th1/Th2 ratio, leukotrines B4 (LTB4) and C4 (LTC4), eosinophil cationic protein (ECP), myeloperoxidase (MPO), and histamine in blood, and intracellular IFN-γ and IL-4 in peripheral blood mononuclear cells. Results: The IAR was accompanied by increased eosinophil and basophil counts, increased serum concentrations of histamine, LTC4 and ECP, decreased Th1/Th2 ratio in favour of Th2 cells, and in- creased intracellular IL-4. The LAR was associated with increased eosinophil and neutrophil counts, increased serum concentrations of LTC4 and LTB4, unchanged Th1/Th2 ratio, and increased intracellular IL-4. The DYAR was accom- panied by increased total leukocyte, neutrophil, monocyte, lymphocyte and thrombocyte counts, increased serum con- centrations of LTB4 and MPO, increased Th1/Th2 ratio in favour of Th1 cells, and increased intracellular IFN-γ. Co