季节性冻结初期川西亚高山高山森林土壤细菌多样性.pdfVIP

生态学报2010 ， 30 (20) :5687-5694 Acta Ecologica Sinica 季节性冻结初期川西亚高山/高山森林 土壤细菌多样性 刘利，吴福忠，杨万勤\王臭，谭泼，余胜 (四川农业大学林学院，四川雅安 625014) 摘要:高山/亚高山显著的季节性冻结过程可能对土壤细菌多样性产生重要影响。为了解季节性冻结初期土壤完全冻结前后川 西亚高山/高山森林群落土壤细菌多样性变化特征，于2008 年 11 月 5 日(土壤冻结前期)一11 月 25 日(土壤完全冻结期)期 间，采用 PCR-DGGE 技术同步研究了原始冷杉(Abies faxoniana )林( PF) 、针阔混交林(MF) 和次生冷杉林(SF) 的土壤细菌群落 多样性变化特征。土壤完全冻结后，3 个森林群落仍然具有较高的土壤细菌多样性。 3 个森林的土壤细菌类群总数在土壤冻结 前表现为 MF SF 町，但在土壤完全冻结后表现为 PF MF SF。土壤冻结明显降低了土壤细菌多样性，但提高了土壤细 菌群落的优势度。冻结作用对土壤细菌群落的影响随着土壤深度增加而降低，随着海拔升高而降低。这些结果表明季节性冻 结过程对亚高山/高山森林土壤细菌多样性有着显著的影响，这对深入认识冬季土壤生态过程具有重要意义。 关键词:季节性冻结;亚高山/高山森林;土壤微生物多样性; DGGE Soil bacterial diversity in the subalpine/alpine forests of western Sichuan at the early stage of freeze-thaw season LIU Li , WU Fuzhong , YANG Wanqin * , W ANG Ao , TAN Bo , YU Sheng Faculty 01 Fores町， Sichuan Agricultural University , Y.ααn 625014 , China Abstract: Significant seasonal soil freezing and thawing could influence greatly on soil bacterial diversity in the alpine/ subalpine forest. To understand the characteristics and dynamics of soil bacterial diversity in the alpine and subalpine forests of westem Sichuan during one onset of soil freezing , a field experiment was conducted to investigate the soil bacterial diversity by PCR-DGGE in the prim缸y fir (Abies faxoniαnα) forest , fir and birch (Betulααlbosinensis) mixed forest and secondary fir (A. faxoT山na) forest , which were three representative forests in the subalpine and alpine forest region in westem Sichuan. Soil samples were collected on Nov.5 and on Nov. 25 , 2008 , which was defined as the onset of freezing based on the simultaneous monitoring of soil temperature. Relative rich soil bacterial groups were still observed in the three sampled forests