基于油茶组59万条EST序列的转录组学初步分析.pdfVIP

林 科 学 第 47 卷第2 期 业 Vol. 47 ,No. 2 2011 年 2 月 SCIENTlA SILVAE SINICAE Feb. ,201 1 基于油茶组59 万条 EST 序列的转录组学初步分析* 陈 英l 江香梅2 张 在 3 张新叶4 项东云5 温强1 ， 2 江聪1 王光萍1 黄敏仁1 徐立安1 (1.南京林业大学南京 21ω37; 2. 江西省林业科学院 南昌 33ω32; 3. 江西农业大学陶揭 33∞45; 4. 湖北省林业科学研究院武汉 43oo79j 5. 广西林业科学研究院南宁 53仰02) 关键诩: 捕茶;转录组; 454 GS FLX; 表达序列标签 中阁分类4号: S718. 46;Q943. 2 文献标识码:A 文章编串串 :1001 -7488(2011)侃 -0161 -03 Traoscriptome Characterizatioo for Camelli归 Sect. Oleifera Based 00 the 592 499 ESTs l 2 l 4 Chen Ying Jiang Xiangmei Zhang Lu Zhang Xinye Xiang Dongyun Wen Qiangl •2 Jiang Congl Wang Guangpingl Huang Minren l Xu Li an l (1. Nanjing Fores町 University Nanjing 210037; 2. Jia~刷i Academy 01 Foreltry Na时hang 330032; 3. Jiangxi Agricullural UnilJlmity Nanch叫33ω45; 4. Hubei Academy 01 Fore$lry Wuhøn 430079; 5. Guangxi Academy 01 Forellry Nønning 53∞02) Abstract: To investigate gene diversity and expression pattem in Cα附II归 Sect. Oleifera , 454 GS FLX platform and TitanÎltm regent were used to produce expressed sequence lags (ESTs) from flower buds of 3 Camellia species. A total of 5 920 499 ESTs were generated. Clustering those sequences twice by Cap3 pr例!uced 15 733 contigs and 20 606 singlets in C. oleifera , 19 397 conligs and 26 882 singlels in C. chekiangoleosa , 14 779 contigs and 25 389 singlets in C. hrevistyla. The GC gradients in 3 transcriplomes basically followed a normal dislribution. In additional all contigs and singlets were functionally categorized into GO ( Gene Onto