DIGE Proteome Analysis Reveals Suitability of Ischemic Cardiac In Vitro Model for Studying Cellular Response to Acute Ischemia and Regeneration 英文参考文献.docVIP

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DIGE Proteome Analysis Reveals Suitability of Ischemic Cardiac In Vitro Model for Studying Cellular Response to Acute Ischemia and Regeneration 英文参考文献.doc

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DIGE Proteome Analysis Reveals Suitability of Ischemic Cardiac In Vitro Model for Studying Cellular Response to Acute Ischemia and Regeneration 英文参考文献

DIGEProteomeAnalysisRevealsSuitabilityofIschemic CardiacInVitroModelforStudyingCellularResponseto AcuteIschemiaandRegeneration SinaHaas1,Heinz-GeorgJahnke1,NoraMoerbt2,MartinvonBergen2,3,SeyedhosseinAharinejad4,5 , OlenaAndrukhova5,6,AndreaA.Robitzki1* 1DivisionofMolecularBiological-BiochemicalProcessingTechnology,CenterforBiotechnologyandBiomedicine,Universita¨tLeipzig,Leipzig,Germany,2Departmentof Proteomics, Helmholtz Centre for Environmental Research, Leipzig, Germany, 3Department of Metabolomics, Helmholtz Centre for Environmental Research, Leipzig, Germany,4DepartmentofCardiacSurgery,CenterofAnatomyandCellBiology,MedicalUniversityofVienna,Vienna,Austria,5DepartmentforCardiovascularResearch, Center of Anatomy and Cell Biology, Medical University of Vienna, Vienna, Austria, 6Department for Biomedical Sciences, Institute of Pathophysiology, University of VeterinaryMedicine,Vienna,Austria Abstract Proteomic analysis of myocardial tissue from patient population is suited to yield insights into cellular and molecular mechanismstakingplaceincardiovasculardiseases.However,ithasbeenlimitedbysmallsizedbiopsiesandcomplicated byhighvariancesbetweenpatients.Therefore,thereisahighdemandforsuitablemodelsystemswiththecapabilityto simulate ischemic and cardiotoxic effects in vitro, under defined conditions. In this context, we established an in vitro ischemia/reperfusion cardiac disease model based on the contractile HL-1 cell line. To identify pathways involved in the cellularalterationsinducedbyischemiaandtherebydefiningdisease-specificbiomarkersandpotentialtargetstructuresfor new drug candidates we used fluorescence 2D-difference gel electrophoresis. By comparing spot density changes in ischemicandreperfusionsampleswedetectedseveralproteinspotsthatweredifferentiallyabundant.UsingMALDI-TOF/ TOF-MSandESI-MStheproteinswereidentifiedandsubsequentlygroupedbyfunctionality.Mostprominentwerechanges in apoptosis signalling, cell structure and energy-metabolism. Alterations were co