Efficient Removal of Platelets from Peripheral Blood Progenitor Cell Products Using a Novel Micro-Chip Based Acoustophoretic Platform 英文参考文献.docVIP

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Efficient Removal of Platelets from Peripheral Blood Progenitor Cell Products Using a Novel Micro-Chip Based Acoustophoretic Platform 英文参考文献.doc

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Efficient Removal of Platelets from Peripheral Blood Progenitor Cell Products Using a Novel Micro-Chip Based Acoustophoretic Platform 英文参考文献

EfficientRemovalofPlateletsfromPeripheralBlood ProgenitorCellProductsUsingaNovelMicro-ChipBased AcoustophoreticPlatform JosefinaDykes1,2*,AndreasLenshof3,Ing-BrittAstrand-Grundstro? ¨m2,ThomasLaurell3,5 ,Stefan Scheding2,4 1Clinical Immunology and Transfusion Medicine, Regional and University Laboratories, Lund, Sweden, 2Stem Cell Center, Institution of Laboratory Medicine, Lund University, Lund, Sweden, 3Measurement Technology and Industrial Electrical Engineering, Lund University, Lund, Sweden, 4Department of Hematology, University HospitalSkane,Lund,Sweden,5BiomedicalEngineering,DonggukUniversity,Seoul,Korea Abstract Background:Excessivecollectionofplateletsisanunwantedsideeffectincurrentcentrifugation-basedperipheralblood progenitorcell(PBPC)apheresis.Weinvestigatedanovelmicrochip-basedacoustophoresistechnique,utilizingultrasonic standingwaveforcesfortheremovalofplateletsfromPBPCproducts.Byapplyinganacousticstandingwavefieldontoa continuouslyflowingcellsuspensioninamicrochannel,cellscanbeseparatedfromthesurroundingmediadependingon theirphysicalproperties. StudyDesignandMethods:PBPCsampleswereobtainedfrompatients(n=15)andhealthydonors(n=6)andsortedon anacoustophoresis-chip.Theacousticforcewassettoseparateleukocytesfromplateletsintoatargetfractionandawaste fraction, respectively. The PBPC samples, the target and the waste fractions were analysed for cell recovery, purity and functionality. Results:Themedianseparationefficiencyofleukocytestothetargetfractionwas98%whereasplateletswereeffectively depletedby89%.PBPCsamplesandcorrespondingtargetfractionsweresimilarinthepercentageofCD34+hematopoetic progenitor/stemcellsaswellasleukocyte/lymphocytesubsetdistributions.Medianviabilitywas98%,98%and97%inthe PBPCsamples,thetargetandthewastefractions,respectively.Resultsfromhematopoieticprogenitorcellassaysindicated apreservedcolony-formingabilitypost-sorting.EvaluationofplateletactivationbyP-selectin(CD62P)expressionrevealeda significantincreaseofCD62P+platel