Effects of Non-Thermal Plasma on Mammalian Cells 英文参考文献.docVIP

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Effects of Non-Thermal Plasma on Mammalian Cells 英文参考文献.doc

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Effects of Non-Thermal Plasma on Mammalian Cells 英文参考文献

EffectsofNon-ThermalPlasmaonMammalianCells SameerKalghatgi1,CrystalM.Kelly2,EkaterinaCerchar3,BehzadTorabi2,OlegAlekseev2 ,Alexander Fridman4,GaryFriedman1,JaneAzizkhan-Clifford2* 1Department of Electrical and Computer Engineering, Drexel University, Philadelphia, Pennsylvania, United States of America, 2Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, Philadelphia, Pennsylvania, United States of America, 3Department of Surgery, Drexel University College of Medicine,Philadelphia,Pennsylvania,UnitedStatesofAmerica,4DepartmentofMechanicalEngineeringandMechanics,DrexelUniversity,Philadelphia,Pennsylvania, UnitedStatesofAmerica Abstract Thermal plasmas and lasers have been widely used in medicine to cut, ablate and cauterize tissues through heating; in contrast,non-thermalplasmaproducesnoheat,soitseffectscanbeselective.Inordertoexploitthepotentialforclinical applications,includingwoundhealing,sterilization,bloodcoagulation,andcancertreatment,amechanisticunderstanding oftheinteractionofnon-thermalplasmawithlivingtissuesisrequired.Usingmammaliancellsinculture,itisshownhere that non-thermal plasma created by dielectric barrier discharge (DBD) has dose-dependent effects that range from increasing cell proliferation to inducing apoptosis. It is also shown that these effects are primarily due to formation of intracellularreactiveoxygenspecies(ROS).Wehaveutilizedc-H2AXtodetectDNAdamageinducedbynon-thermalplasma andfoundthatitisinitiatedbyproductionofactiveneutralspeciesthatmostlikelyinduceformationoforganicperoxides in cell medium. Phosphorylation of H2AX following non-thermal plasma treatment is ATR dependent and ATM independent,suggestingthatplasmatreatmentmayleadtoreplicationarrestorformationofsingle-strandedDNAbreaks; however,plasmadoesnotleadtoformationofbulkyadducts/thyminedimers. Citation: Kalghatgi S, Kelly CM, Cerchar E, Torabi B, Alekseev O, et al. (2011) Effects of Non-Thermal Plasma on Mammalian Cells. PLoS ONE 6(1): e1627