Molecular Characterization of Pneumococcal Isolates from Pets and Laboratory Animals 英文参考文献.docVIP

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Molecular Characterization of Pneumococcal Isolates from Pets and Laboratory Animals 英文参考文献.doc

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Molecular Characterization of Pneumococcal Isolates from Pets and Laboratory Animals 英文参考文献

MolecularCharacterizationofPneumococcalIsolates fromPetsandLaboratoryAnimals MarkvanderLinden1*,AdnanAl-Lahham2,WernerNicklas3,RalfRene′ Reinert1¤ 1DepartmentofMedicalMicrobiology,NationalReferenceCenterforStreptococci,UniversityHospital(RWTH),Aachen,Germany,2SchoolofAppliedMedicalSciences, German-JordanianUniversity,Amman,Jordan,3GermanCancerResearchCentre,Heidelberg,Germany Abstract Background: Between 1986 and 2008 Streptococcus pneumoniae was isolated from 41 pets/zoo animals (guinea pigs (n=17), cats (n=12), horses (n=4), dogs (n=3), dolphins (n=2), rat (n=2), gorilla (n=1)) treated in medical veterinary laboratoriesandzoos,and44laboratoryanimals(mastomys(multimammatemice;n=32),mice(n=6),rats(n=4),guinea pigs (n=2)) during routine health monitoring in an animal facility. S. pneumoniae was isolated from nose, lung and respiratorytract,eye,earandothersites. Methodology/Principal Findings: Carriage of the same isolate of S. pneumoniae over a period of up to 22 weeks was shownforfourmastomys. Forty-one animals showed disease symptoms. Pneumococcal isolates werecharacterized by optochin sensitivity, bile solubility, DNA hybridization, pneumolysin PCR, serotyping and multilocus sequence typing. Eighteen of the 32 mastomys isolates (56%) were optochin resistant, all other isolates were optochin susceptible. All mastomysisolateswereserotype14,allguineapigisolatesserotype 19F,allhorseisolatesserotype3.Ratshadserotypes 14 or 19A, mice 33A or 33F. Dolphins had serotype 23F, the gorilla serotype 14. Cats and dogs had many different serotypes. Four isolates were resistant to macrolides, three isolates also to clindamycin and tetracyclin. Mastomys isolatesweresequencetype(ST)15(serotype 14),anST/serotype combination commonlyfoundinhumanisolates. Cats, dogs, pet rats, gorilla and dolphins showed various human ST/serotype combinations. Lab rats and lab mice showed single locusvariants (SLV)ofhumanSTs,inhumanST/serotype combinations. Allguinea pigisolates sho