Optimizing the Extraction of Anti-tumor Polysaccharides from the Fruit of Capparis spionosa L. by Response Surface Methodology 英文参考文献.docVIP

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Optimizing the Extraction of Anti-tumor Polysaccharides from the Fruit of Capparis spionosa L. by Response Surface Methodology 英文参考文献.doc

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Optimizing the Extraction of Anti-tumor Polysaccharides from the Fruit of Capparis spionosa L. by Response Surface Methodology 英文参考文献

Molecules 2012, 17, 7323-7335; doi:10.3390/moleculeOPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Article Optimizing the Extraction of Anti-tumor Polysaccharides from the Fruit of Capparis spionosa L. by Response Surface Methodology Yu-Bin Ji 1,*, Fang Dong 1, Dong-Bin Ma 2, Jing Miao 2, Li-Na Jin 2, Zhen-Feng Liu 1 and Ling-Wen Zhang 1 1 Research Center on Life Sciences and Environmental Sciences, Harbin University of Commerce, Harbin 150076, China; E-Mails: dong1234fang@126.com (F.D.); huawuxinde@163.com (Z.-F.L.); lingwen2008@163.com (L.-W.Z.) 2 Engineering Research Center of Natural Anticancer Drugs, Ministry of Education, Harbin 150076, China; E-Mails: madongbin1987@163.com (D.-B.M.); miaojing586@163.com (J.M.); kinglena@126.com (L.-N.J.) * Author to whom correspondence should be addressed; E-Mail: jyb@; Tel.: +86-451-8480-0297; Fax: +86-451-8484-4417. Received: 1 April 2012; in revised form: 5 June 2012 / Accepted: 11 June 2012 / Published: 14 June 2012 Abstract: Capparis spionosa L. is a traditional medicinal plant in China and central Asia. In this study, an experiment was designed to investigate the optimization of the extraction of anti-tumor polysaccharides from the fruit of Capparis spionosa L. (CSPS) by response surface methodology (RSM). Four independent variables (extraction temperature, extraction time, ratio of water to sample and extraction cycles) were explored. Meanwhile, the in vivo anti-tumor activity of CSPS was investigated. The results showed that the experimental data could be fitted to a second-order polynomial equation using multiple regression analysis. The optimum extraction conditions were as follows: extraction temperature 92 °C, extraction time 140 min, ratio of water to sample 26 mL/g, and three extraction cycle. Under these conditions, the yield of polysaccharides reached 13.01%, which was comparable to the predicted yield (12.94%