Probiotic Bacteria Regulate Intestinal Epithelial Permeability in Experimental Ileitis by a TNF-Dependent Mechanism 英文参考文献.docVIP

下载本文档

1
0
约5.23万字
约 10页
2017-05-12 发布于上海
举报
版权申诉

Probiotic Bacteria Regulate Intestinal Epithelial Permeability in Experimental Ileitis by a TNF-Dependent Mechanism 英文参考文献.doc

1、本文档共10页，可阅读全部内容。
2、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。
3、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
5、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
6、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
7、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
8、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

Probiotic Bacteria Regulate Intestinal Epithelial Permeability in Experimental Ileitis by a TNF-Dependent Mechanism 英文参考文献

ProbioticBacteriaRegulateIntestinalEpithelial PermeabilityinExperimentalIleitisbyaTNF-Dependent Mechanism DanieleCorridoni2,LucaPastorelli1,BenedettaMattioli1,SilviuLocovei1,2,DaiIshikawa2, KristenO.Arseneau2,MarcelloChieppa3,FabioCominelli2.,TheresaT.Pizarro1*. 1Department ofPathology, CaseWesternReserveUniversitySchool ofMedicine,Cleveland,Ohio,United States ofAmerica, 2Division ofGastroenterology andLiver Disease, Department of Medicine, Case Western Reserve University School of Medicine, Cleveland, Ohio, United States of America, 3Lab of Experimental Immunopathology,InstitutodiRicoveroeCuraaCarattereScientifico(IRCCS)‘‘DeBellis’’,CastellanaGrotte,Bari,Italy Abstract Background:Wepreviouslyshowedthattheprobioticmixture,VSL#3,preventstheonsetofileitisinSAMP/YitFc(SAMP) mice,andthiseffectwasassociatedwithstimulationofepithelial-derivedTNF.Theaimofthisstudywastodeterminethe mechanism(s) of VSL#3-mediated protection on epithelial barrier function and to further investigate the ‘‘paradoxical’’ effectsofTNFinpreventingSAMPileitis. Methods:PermeabilitywasevaluatedinSAMPmicepriortotheonsetofinflammationandduringestablisheddiseaseby measuring transepithelial electrical resistance (TEER) on ex vivo-cultured ilea following exposure to VSL# 3 conditioned media (CM), TNF or VSL#3-CM + anti-TNF. Tight junction (TJ) proteins were assessed by qRT-PCR, Western blot, and confocalmicroscopy,andTNFRI/TNFRIIexpressionmeasuredinfreshlyisolatedintestinalepithelialcells(IEC)fromSAMP andcontrolAKRmice. Results:CulturewitheitherVSL#3-CMorTNFresultedindecreasedilealparacellularpermeabilityinpre-inflamedSAMP, butnotSAMPwithestablisheddisease,whileadditionofanti-TNFabrogatedtheseeffects.ModulationoftheTJproteins, claudin-2andoccludin,occurredwithasignificantdecreaseinclaudin-2andincreaseinoccludinfollowingstimulationwith VSL#3-CM or TNF. TNF protein levels increased in supernatants of SAMP ilea incubated with VSL#3-CM compared to vehicle, while IEC-derived TNFR mRNA expression decrea