Production of polyhydroxyalkanoates from waste frying oil by Cupriavidus necator 英文参考文献.docVIP
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Production of polyhydroxyalkanoates from waste frying oil by Cupriavidus necator 英文参考文献
Verlindenetal.AMBExpress2011,1:11
/content/1/1/11
ORIGINAL
OpenAccess
Productionofpolyhydroxyalkanoatesfromwaste
fryingoilbyCupriavidusnecator
RobAJVerlinden1,DavidJHill1,MelvinAKenward1,CraigDWilliams1,ZofiaPiotrowska-Seget2and
IzaKRadecka1*
Abstract
Polyhydroxyalkanoates(PHAs)arebiopolymers,whichcanreplacepetrochemicalplasticsinmanyapplications.
However,thesebioplasticsarecurrentlyfarmoreexpensivethanpetrochemicalplastics.Manyresearchersare
investigatingtheuseofinexpensivesubstratesderivedfromwastestreams.Wastefryingoilisabundantandcan
beusedinPHAproductionwithoutfiltration.
Cupriavidusnecator(formerlyknownasRalstoniaeutropha)isaversatileorganismfortheproductionofPHAs.
Small-scalebatchfermentationstudieshavebeensetup,usingdifferentconcentrationsofpurevegetableoil,
heatedvegetableoilandwastefryingoil.Theseoilsareallrapeseedoils.
IthasbeenshownthatCupriavidusnecatorproducedthehomopolymerpolyhydroxybutyrate(PHB)fromthe
rapeseedoils.TheachievedPHBconcentrationfromwastefryingoilwas1.2g/l,whichissimilartoaconcentration
thatcanbeobtainedfromglucose.ThePHBharvestfrompureoilandheatedoilwas0.62g/land0.9g/l
respectively.Afeedofwastefryingoilcouldthusachievemorebiopolymerthanpurevegetableoil.Whiletheuse
ofawasteproductisbeneficialfromalife-cycleperspective,PHBisnottheonlyproductthatcanbemadefrom
wasteoil.Thecollectionofwastefryingoilisbecomingmorewidespread,makingwasteoilagoodalternativeto
purifiedoilorglucoseforPHBproduction.
Keywords:polyhydroxyalkanoates,polyhydroxybutyrate,bacterialfermentation,biopolymer,wastefryingoil,rape-
seedoil
Introduction
investigated thoroughly (Lemosetal. 2006,; Castilho et
Polyhydroxyalkanoates (PHAs) are natural, renewable al.2009).
and biocompatible biopolymers, produced intracellular
Vegetable oils have been found to be possible sub-
in bacteria. They can be made into plastic materials stratesintheproductionofPHAs(AliasandTan2005,;
withpropertiesthataresimilartopetrochemicalplastics Kaharetal.2004,).Wastestreamsfromoilmillsorused
and
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