Rapid Identification of ESKAPE Bacterial Strains Using an Autonomous Microfluidic Device 英文参考文献.docVIP

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Rapid Identification of ESKAPE Bacterial Strains Using an Autonomous Microfluidic Device 英文参考文献.doc

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Rapid Identification of ESKAPE Bacterial Strains Using an Autonomous Microfluidic Device 英文参考文献

RapidIdentificationofESKAPEBacterialStrainsUsingan AutonomousMicrofluidicDevice JackY.Ho1,NateJ.Cira2,JohnA.Crooks3,JosueBaeza3,DouglasB.Weibel1,3* 1Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, Wisconsin, United States of America, 2Department of Bioengineering, Stanford University,Stanford,California,UnitedStatesofAmerica,3DepartmentofBiochemistry,UniversityofWisconsin-Madison,Madison,Wisconsin,UnitedStatesofAmerica Abstract This article describes Bacteria ID Chips (‘BacChips’): an inexpensive, portable, and autonomous microfluidic platform for identifying pathogenic strains of bacteria. BacChips consist of a set of microchambers and channels molded in the elastomericpolymer,poly(dimethylsiloxane)(PDMS).Eachmicrochamberispreloadedwithmono-,di-,ortrisaccharidesand dried.PressingthelayerofPDMSintocontactwithaglasscoverslipformsthedevice;thefootprintofthedeviceinthis article is ,6cm2. After assembly, BacChips are degased under large negative pressure and are stored in vacuum-sealed plasticbags.Tousethedevice,thebagisopened,asamplecontainingbacteriaisintroducedattheinletofthedevice,and thedegasedPDMSdrawsthesampleintothecentralchannelandchambers.Aftertheliquidattheinletisconsumed,airis drawn into the BacChip via the inlet and provides a physical barrier that separates the liquid samples in adjacent microchambers.ApHindicatorisadmixedwiththesamplespriortotheirloading,enablingthemetabolismofthedissolved saccharides in the microchambers to be visualized. Importantly, BacChips operate without external equipment or instruments.Byvisuallydetectingthegrowthofbacteriausingambientlightafter ,4h,wedemonstratethatBacChips withtenmicrochamberscontainingdifferentsaccharidescanreproduciblydetecttheESKAPEpanelofpathogens,including strains of: Enterococcus faecalis, Enteroccocus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Enterobacter aerogenes, and Enterobacter cloacae. This article