Reprogramming of H3K27me3 Is Critical for Acquisition of Pluripotency from Cultured Arabidopsis Tissues 英文参考文献.docVIP

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Reprogramming of H3K27me3 Is Critical for Acquisition of Pluripotency from Cultured Arabidopsis Tissues 英文参考文献.doc

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Reprogramming of H3K27me3 Is Critical for Acquisition of Pluripotency from Cultured Arabidopsis Tissues 英文参考文献

ReprogrammingofH3K27me3IsCriticalforAcquisition ofPluripotencyfromCulturedArabidopsisTissues ChongshengHe,XiaofanChen,HaiHuang,LinXu* National Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology and Ecology, Shanghai Institutesfor Biological Sciences, Chinese Academy of Sciences,Shanghai,China Abstract Inplants,multipledetachedtissuesarecapableofformingapluripotentcellmass,termedcallus,whenculturedonmedia containingappropriateplanthormones.Recentstudiesdemonstratedthatcallusresemblestheroot-tipmeristem,evenifit isderivedfromaerialorgans.Thisfindingimprovesourunderstandingoftheregenerationprocessofplantcells;however, themolecularmechanismthatguidescellsofdifferenttissuetypestoformacallusstillremainselusive.Here,weshowthat genome-wide reprogramming of histone H3 lysine 27 trimethylation (H3K27me3) is a critical step in the leaf-to-callus transition.ThePolycombRepressiveComplex2(PRC2)isknowntofunctioninestablishingH3K27me3.Byanalyzingcallus formation of mutants corresponding to different histone modification pathways, we found that leaf blades and/or cotyledonsofthePRC2mutantscurlyleafswinger(clfswn)andembryonicflower2(emf2)weredefectiveincallusformation. We identified the H3K27me3-covered loci in leaves and calli by a ChIP–chip assay, and we found that in the callus H3K27me3levelsdecreasedfirstatcertainauxin-pathwaygenes.Thelevelswerethenincreasedatspecificleafgenesbut decreasedatanumberofroot-regulatorygenes.ChangesinH3K27me3levelswerenegativelycorrelatedwithexpression levels of the corresponding genes. One possible role of PRC2-mediated H3K27me3 in the leaf-to-callus transition might relate to elimination of leaf features by silencing leaf-regulatory genes, as most leaf-preferentially expressed regulatory genescouldnotbesilencedintheleafexplantsofclfswn.Incontrasttotheleafexplants,therootexplantsofbothclfswn and emf2 formed calli normally, possibly because the root-to-callus transition bypasses the leaf gene silencing process. Furthermor