RNA Methylation by the MIS Complex Regulates a Cell Fate Decision in Yeast 英文参考文献.docVIP

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RNA Methylation by the MIS Complex Regulates a Cell Fate Decision in Yeast 英文参考文献.doc

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RNA Methylation by the MIS Complex Regulates a Cell Fate Decision in Yeast 英文参考文献

RNAMethylationbytheMISComplexRegulatesaCell FateDecisioninYeast SudeepD.Agarwala1,2,HannahG.Blitzblau1,AndreasHochwagen1¤,GeraldR.Fink1,2* 1WhiteheadInstitute,Cambridge,Massachusetts,UnitedStatesofAmerica,2DepartmentofBiology,MassachusettsInstituteofTechnology,Cambridge,Massachusetts, UnitedStatesofAmerica Abstract FortheyeastSaccharomycescerevisiae,nutrientlimitationisakeydevelopmentalsignalcausingdiploidcellstoswitchfrom yeast-formbuddingtoeitherforagingpseudohyphal(PH)growthormeiosisandsporulation.Prolongedstarvationleadsto lineage restriction, suchthat cells exitingmeiotic prophasearecommitted tocomplete sporulation evenif nutrientsare restored.Here,wehaveidentifiedanearliercommitmentpointinthestarvationprogram.Afterthispoint,cells,returnedto nutrient-rich medium, entered a form of synchronous PH development that was morphologically and genetically indistinguishable from starvation-induced PH growth. We show that lineage restriction during this time was, in part, dependent on the mRNA methyltransferase activity of Ime4, which played separable roles in meiotic induction and suppressionofthePHprogram.NormallevelsofmeioticmRNAmethylationrequiredthecatalyticdomainofIme4,aswell astwomeioticproteins,Mum2andSlz1,whichinteractedandco-immunoprecipitatedwithIme4.ThisMIScomplex(Mum2, Ime4,andSlz1)functionedinbothstarvationpathways.Together,ourresultssupportthenotionthattheyeaststarvation response is an extended process that progressively restricts cell fate and reveal abroad role of post-transcriptional RNA methylationinthesedecisions. Citation:AgarwalaSD,BlitzblauHG,HochwagenA,FinkGR(2012)RNAMethylationbytheMISComplexRegulatesaCellFateDecisioninYeast.PLoSGenet8(6): e1002732.doi:10.1371/journal.pgen.1002732 Editor:MichaelLichten,NationalCancerInstitute,UnitedStatesofAmerica ReceivedJanuary18,2012;AcceptedApril10,2012;PublishedJune7,2012 Copyright: ? 2012 Agarwala et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License