RssAB Signaling Coordinates Early Development of Surface Multicellularity in Serratia marcescens 英文参考文献.docVIP

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RssAB Signaling Coordinates Early Development of Surface Multicellularity in Serratia marcescens 英文参考文献.doc

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RssABSignalingCoordinatesEarlyDevelopmentof SurfaceMulticellularityinSerratiamarcescens Yu-HuanTsai1,Jun-RongWei2,Chuan-ShengLin3,Po-HanChen1,StellaHuang1,Yu-ChingLin4 ,Chia- FongWei4,Chia-ChenLu5,Hsin-ChihLai4* 1DepartmentofClinicalLaboratorySciencesandMedicalBiotechnology,NationalTaiwanUniversityCollegeofMedicine,Taipei,Taiwan,RepublicofChina,2Department ofImmunologyandInfectiousDiseases,HarvardSchoolofPublicHealth,Boston,Massachusetts,UnitedStatesofAmerica,3DepartmentofBiochemistryandMolecular Biology,ChangGungUniversity,Kweishan,Taoyuan,Taiwan,RepublicofChina,4DepartmentofMedicalBiotechnologyandLaboratoryScience,andResearchCenterfor PathogenicBacteria,ChangGungUniversity,Kwei-Shan,Tao-Yuan,Taiwan,RepublicofChina,5DepartmentofRespiratoryTherapy,FuJenCatholicUniversity,Sinjhuang, Taipei,Taiwan,RepublicofChina Abstract Bacteriacancoordinateseveralmulticellularbehaviorsinresponsetoenvironmentalchanges.Amongthese,swarmingand biofilm formation have attracted significant attention for their correlation with bacterial pathogenicity. However, little is knownaboutwhenandwherethesignalingoccurstotriggereitherswarmingorbiofilmformation.Wehavepreviously identifiedanRssABtwo-componentsysteminvolvedintheregulationofswarmingmotilityandbiofilmformationinSerratia marcescens.HerewemonitoredtheRssAB signalingstatuswithin singlecellsby tracingthelocationofthetranslational fusionproteinEGFP-RssBfollowingdevelopmentofswarmingorbiofilmformation.RssABsignalingisspecificallyactivated beforesurfacemigrationinswarmingdevelopmentandduringtheearlystageofbiofilmformation.Theactivationresultsin thereleaseofRssBfromitscognateinnermembranesensorkinase,RssA,tothecytoplasmwherethedownstreamgene promoters are located. Such dynamic localization of RssB requires phosphorylation of this regulator. By revealing the temporalactivationofRssABsignalingfollowingdevelopmentofsurfacemulticellularbehavior,ourfindingscontributeto animprovedunderstandingofhowbacteriacoordinatetheirlifestyleonasurface. Cit