RsfA (YbeB) Proteins Are Conserved Ribosomal Silencing Factors 英文参考文献.docVIP

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RsfA (YbeB) Proteins Are Conserved Ribosomal Silencing Factors 英文参考文献.doc

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RsfA(YbeB)ProteinsAreConservedRibosomalSilencing Factors RomanHa¨user1,2.,MarkusPech3,4.,JaroslawKijek3,4.,HiroshiYamamoto3,4.,Bjo¨rnTitz1¤, FlorianNaeve1,AndreyTovchigrechko5,KaoriYamamoto3,4,WitoldSzaflarski3,6,NonoTakeuchi7, ThorstenStellberger1,MarkusE.Diefenbacher1,KnudH.Nierhaus3,4*,PeterUetz1,8,9 * 1Institute of Toxicology and Genetics, Karlsruhe Institute of Technology (KIT), Karlsruhe, Germany, 2German Cancer Research Center (DKFZ), Heidelberg, Germany, 3Abteilung AG Ribosomen Max-Planck-Institut fu¨r Molekulare Genetik, Berlin, 4Institut fu¨r Medizinische Physik und Charite′– Vingron, Germany, Biophysik, Universita¨tsmedizin Berlin,Berlin,Germany,5J.CraigVenterInstitute(JCVI),Rockville,Maryland,UnitedStatesofAmerica,6DepartmentofHistologyandEmbryology, PoznanUniversityofMedicalSciences,Poznan,Poland,7DepartmentofMedicalGenomeSciences,GraduateSchoolofFrontierSciences,UniversityofTokyo,Kashiwa-shi, Chiba, Japan, 8Proteros Biostructures, Martinsried, Germany, 9Center for the Study of Biological Complexity, Virginia Commonwealth University, Richmond, Virginia, UnitedStatesofAmerica Abstract TheYbeB(DUF143)familyofuncharacterizedproteinsisencodedbyalmostallbacterialandeukaryoticgenomesbutnot archaea.Whiletheyhavebeenshowntobeassociatedwithribosomes,theirmolecularfunctionremainsunclear.Herewe showthatYbeBisaribosomalsilencingfactor(RsfA)inthestationarygrowthphaseandduringthetransitionfromrichto poormedia. A knock-outof the rsfA geneshows twostrong phenotypes: (i)the viability ofthemutant cells aresharply impaired during stationary phase (as shown by viability competition assays), and (ii) during transition from rich to poor mediathemutantcellsadaptslowlyandshowagrowthblockofmorethan10hours(asshownbygrowthcompetition assays). RsfA silences translation by binding to the L14 protein of the large ribosomal subunit and, as a consequence, impairssubunitjoining(asshownbymolecularmodeling,reporter geneanalysis, invitrotranslationassays,andsucrose gradient