Screening a mushroom extract library for activity against Acinetobacter baumannii and Burkholderia cepacia and the identification of a compound with anti-Burkholderia activity 英文参考文献.docVIP

下载本文档

3
0
约2.95万字
约 6页
2017-05-13 发布于上海
举报
版权申诉

Screening a mushroom extract library for activity against Acinetobacter baumannii and Burkholderia cepacia and the identification of a compound with anti-Burkholderia activity 英文参考文献.doc

1、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。。
2、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
4、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
5、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
6、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
7、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

Screening a mushroom extract library for activity against Acinetobacter baumannii and Burkholderia cepacia and the identification of a compound with anti-Burkholderia activity 英文参考文献

Schwanetal.AnnalsofClinicalMicrobiologyandAntimicrobials2010,9:4 /content/9/1/4 RESEARCH OpenAccess Screeningamushroomextractlibraryforactivity againstAcinetobacterbaumanniiandBurkholderia cepaciaandtheidentificationofacompound withanti-Burkholderiaactivity WilliamRSchwan1*,CraigDunek1,2,MichaelGebhardt1,KathleenEngelbrecht1,TiffanyKlett1,AaronMonte2, JosephToce2,MarcRott1,ThomasJVolk3,JohnJLiPuma4,Xue-TingLiu2,RonaldMcKelvey2 Abstract Background:AcinetobacterbaumanniiandspecieswithintheBurkholderiacepaciacomplex(BCC)aresignificant opportunisticbacterialpathogensofhumans.Thesespeciesexhibitahighdegreeofantibioticresistance,and someclinicalisolatesareresistanttoallcurrentlyavailableantimicrobialdrugsusedfortreatment.Thus,newdrugs areneededtotreatinfectionsbythesespecies.MushroomscouldbeapotentialsourcefornewdrugstotreatA. baumanniiandBCCinfections. Methods:Theaimofthisstudywastoscreenalibraryofcrudeextractsfrom330wildmushroomsbydisk diffusionassaysforantibacterialactivityagainstA.baumanniiandBurkholderiacepaciainthehopeofidentifyinga novelnaturaldrugthatcouldbeusedtotreatinfectionscausedbythesespecies.Oncepositivehitswere identified,theextractsweresubjectedtobioassay-guidedseparationstoisolateandidentifytheactivedrug molecules.MICswereperformedtogaugetheinvitroactivityofthepurifiedcompounds. Results:Onlythreecrudeextracts(0.9%)hadactivityagainstA.baumanniiandB.cepacia.Compoundsfromtwoof theseextractshadMICsgreaterthan128μg/ml,andfurtheranalyseswerenotperformed.Fromthethirdextract, preparedfromLeucopaxillusalbissimus,2-aminoquinoline(2-AQ)wasisolated.Thiscompoundexhibitedamodest MICinvitroagainststrainsfromninedifferentBCCspecies,includingmulti-drugresistantclinicalisolates(MIC=8- 64μg/ml),andaweakMIC(128μg/ml)againstAbaumannii.TheIC50 againstamurinemonocytelinewas1.5mg/ ml. Conclusion:Thesmallnumberofpositivehitsinthisstudysuggeststhatfindinganewdrugfrommushroomsto treatGram-negativebacterialinfectionsmaybedifficult.Although2-AQwasidentifiedinonemushroom,andi