Screening of 71 P. multocida Proteins for Protective Efficacy in a Fowl Cholera Infection Model and Characterization of the Protective Antigen PlpE 英文参考文献.docVIP

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Screening of 71 P. multocida Proteins for Protective Efficacy in a Fowl Cholera Infection Model and Characterization of the Protective Antigen PlpE 英文参考文献.doc

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Screening of 71 P. multocida Proteins for Protective Efficacy in a Fowl Cholera Infection Model and Characterization of the Protective Antigen PlpE 英文参考文献

Screeningof71P.multocidaProteinsforProtective EfficacyinaFowlCholeraInfectionModeland CharacterizationoftheProtectiveAntigenPlpE Tama′sHatfaludi2*.,KeithAl-Hasani1,3.¤,LanGong1,2,JohnD.Boyce1,3,MarkFord4,IanW.Wilkie5, NoeleneQuinsey1,2,MichelleA.Dunstone2,3,DavidE.Hoke2,BenAdler1,3 1Australian Research Council Centre of Excellence in Structural and Functional Microbial Genomics, Monash University, Clayton, Victoria, Australia, 2Department of BiochemistryandMolecularBiology,MonashUniversity,Clayton,Victoria,Australia,3DepartmentofMicrobiology,MonashUniversity,Clayton,Victoria,Australia,4CSIRO Livestock Industries, Australian Animal Health Laboratory, Geelong, Victoria, Australia, 5Veterinary Pathology and Anatomy, University of Queensland, Brisbane, Queensland,Australia Abstract Background:Thereisastrongneedforarecombinantsubunitvaccineagainstfowlcholera.Weusedareversevaccinology approachtoidentifyputativesecretedorcellsurfaceassociatedP.multocidaproteinsthatmayrepresentpotentialvaccine candidateantigens. Principal Findings: A high-throughput cloning and expression protocol was used to express and purify 71 recombinant proteinsforvaccinetrials.Ofthe71proteinstested,onlyone,PlpEindenaturedinsolubleform,protectedchickensagainst fowl cholera challenge. PlpE also elicited comparable levels of protection in mice. PlpE was localized by immunofluorescence to the bacterial cell surface, consistent with its ability to elicit a protective immune response. To exploretheroleofPlpEduringinfectionandimmunity,aplpEmutantwasgenerated.TheplpEmutantstrainretainedfull virulenceformice. Conclusion:ThesestudiesshowthatPlpEisasurfaceexposedproteinandwastheonlyproteinof71testedthatwasable to elicit a protective immune response. However, PlpE is not an essential virulence factor. This is the first report of a denaturedrecombinantproteinstimulatingprotectionagainstfowlcholera. Citation:HatfaludiT,Al-HasaniK,GongL,BoyceJD,FordM,etal.(2012)Screeningof71P.multocidaProteinsforProtectiveEf