Secondary Chromosomal Attachment Site and Tandem Integration of the Mobilizable Salmonella Genomic Island 1 英文参考文献.docVIP

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Secondary Chromosomal Attachment Site and Tandem Integration of the Mobilizable Salmonella Genomic Island 1 英文参考文献.doc

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Secondary Chromosomal Attachment Site and Tandem Integration of the Mobilizable Salmonella Genomic Island 1 英文参考文献

SecondaryChromosomalAttachmentSiteandTandem IntegrationoftheMobilizableSalmonellaGenomic Island1 Beno??tDoublet1*,GeorgeR.Golding2,MichaelR.Mulvey2,AxelCloeckaert1 1INRA, UR1282, Infectiologie Animale et Sante′ Publique, Nouzilly, France, 2National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada Abstract Background:TheSalmonellagenomicisland1isanintegrativemobilizableelement(IME)originallyidentifiedinepidemic multidrug-resistantSalmonella entericaserovarTyphimurium(S.Typhimurium)DT104.SGI1containsacomplexintegron, whichconfersvariousmultidrugresistancephenotypesduetoitsgeneticplasticity.PreviousstudieshaveshownthatSGI1 integratessite-specificallyintotheS.enterica,Escherichiacoli,orProteusmirabilischromosomeatthe39endofthdFgene (attBsite). Methodology/Principal Findings: Here, wereport thetransferof SGI1toa DthdF mutantof S.Typhimurium LT2. Inthe absenceofthdF,thefrequencyoftransconjugantformationwasreducedbyaroundthirtytimesofmagnitude.Through DNAsequencingSGI1wasshowntointegratespecificallyintoasecondaryattachmentsite(2ndattB),whichislocatedinthe intergenicregionbetweenthechromosomalsodBandpurRgenes.Atthis2ndattBsite,wefoundthatasignificantfraction ofSGI1transconjugants(43%ofwildtypeand100%ofDthdFmutant)containedtandemSGI1arrays.Moreover,inwildtype S.TyphimuriumLT2transconjugants,SGI1integratedintobothattachmentsites,i.e.,thdFandsodB-purR.Theformationof SGI1 tandem arrays occurred in both specific attB sites. There was heterogeneity in the size of the SGI1 tandem arrays detectedinsingletransconjugant colonies.SomearraysconsistedasfarassixSGI1sarrangedintandem.Thesetandem arrayswereshowntopersistduringserialpassageswithorwithoutantibioticselectionpressure. Conclusions/Significance: The ability of integration into two distinct chromosomal sites and tandem array formation of SGI1 could contribute to its spread and persistence. The existence of a secondary attachment site in the Salmonella chromosomehaspotential implications fo