Variability in synovial inflammation in rheumatoid arthritis investigated by microarray technology 英文参考文献.docVIP

Available online /content/8/2/R47 Research article Open Access Vol 8 No 2 Variability in synovial inflammation in rheumatoid arthritis investigated by microarray technology Johan Lindberg1, Erik af Klint2, Ann-Kristin Ulfgren2, André Stark3, Tove Andersson1, Peter Nilsson1, Lars Klareskog2 and Joakim Lundeberg1 1Department of Biotechnology, AlbaNova University Center, Royal Institute of Technology, S-106 91 Stockholm, Sweden 2Department of Rheumatology, Karolinska Institutet, Karolinska University Hospital, 171 76 Stockholm, Sweden 3Department of Orthopedics, Karolinska University Hospital, 171 76 Stockholm, Sweden Corresponding author: Joakim Lundeberg, joakim.lundeberg@biotech.kth.se Received: 3 Jul 2005 Revisions requested: 9 Sep 2005 Revisions received: 18 Nov 2005 Accepted: 23 Jan 2006 Published: 16 Feb 2006 Arthritis Research Therapy 2006, 8:R47 (doi:10.1186/ar1903) This article is online at: /content/8/2/R47 ? 2006 Lindberg et al.; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract In recent years microarray technology has been used biopsies from the same patient was about three times larger in orthopedic than in arthroscopic biopsies. Using a parallel analysis of the tissues by immunohistochemistry, we also identified orthopedic biopsies that were unsuitable for gene expression analysis of synovial inflammation due to sampling of non-inflamed parts of the tissue. Removing these biopsies reduced the average number of differentially expressed genes between the orthopedic biopsies from 455 to 171, in comparison with 143 for the arthroscopic biopsies. Hierarchical clustering analysis showed that the remaining orthopedic and arthroscopic biopsies had gene expression