烟草青枯病病圃土壤微生物多样性初步分析.pdfVIP

烟草科技I Tobacco Science Technology 病虫害防治I Disease Pest Control 2012 年第 8 期(总第 301 期) 烟草青枯病病圃土壤微生物多样性初步分析 蔡刘体l2 ，商胜华，胡重怡，王丽丽丁文IJ 艳霞2 ，汪汉成，杨兴明2 ，石俊雄叫 1.贵州省烟草科学研究所，贵阳市金阳新区云潭北路 550081 2. 南京农业大学资源与环境科学学院，南京市卫岗童卫路 l 号 210095 摘要:为了更好地利用烟草青枯茵拮抗菌防治烟草青枯病，采用微生物保守区域扩增、构建文库、生物信 息比对的方法初步分析了烟草青枯病病因土壤中微生物的多样性。结果显示，烟草青枯病病圆土壤微生 物群落中含有伯克霍尔德氏菌( Burkholderiα fungorum) 、假羊胞杆菌属类 (Pseudomonas graminis )、黑霉菌 (Aspergillus niger )、尖 J包镰刀菌属( Fusarium oxysporum )等丰富的细菌类和真茵类微生物，也包括部分不可 培养的微生物。土壤中微生物多样性的分析可以为青枯茵拮抗菌应用中拮抗菌在土壤中的定殖、扩繁过 程研究提供部分微生物群落信息。 关键词:烟草;土壤;微生物;青枯病;青枯菌 中图分类号: S435.72 文献标识码 :A 文章编号: 1002-0861(2012)08-0079-05 Preliminary Analysis of Soil Microbial Diversity in Tobacco Bacterial Wilt Disease Nursery CAI Liuti 1.2, SHANG Shenghua ,HU Zhongyi , WANG Lili , LIU Yanxia , WANG Hancheng , YANG Xingming2 , and SHI Junxiong 1. Guizhou Tobacco Research Institute , Guiyang 550081 , China 2. CoUege of Resource and Environmental Science. Nanjing Agricultural University , Nanjing 210095 , China Abstract: To prevent and control tobacco bacterial wilt disease with antagonistic bacterium against Rαlstonia solαnαcearum ， th巳 microbial diversity in soil of tobacco bacterial wilt disease nursery was preliminarily analyzed by means of conservative fragment amplification , construction of library and bio-information comparison. The results indicated that there existed abundant microbial communities including not only Burkholderiα ungorum ， Pseudomonαs graminis , Aspergillus niger and Fusαrium oxysporum , but also some uncultivable bacterium in the soil. The analysis of microbial diversity in the soil could provide some informatìon about microbial community structure for the study on colonization and proliferation process in soil in the application of antagonistic bacterium against Rαlstoniα 50lαnαcearum. Keywords: Tobacco; Soil; Microbe; Bacterial wi 1t disease; Rαlstoniα 50lαnacearum