严重烫伤影响家兔外周血中性粒细胞的蛋白质组学研究.pdfVIP

1572 • ·论著· 严嚣烫伤影晌家兔外周血中性粒细胞的蛋白质组学研究僻 曾妃章张3i红李莉莉任利成梁鹏飞黄晓元 (中南大学湘雅医院烧伤整形外科，湖南 长沙 410008) [摘要]【阔的研究严重烫伤对家兔外用此中性根细胞蛋白质级的影响。【方法】12 只家兔分为 A、日两 组(每组 6 只)， A 组为对照组(假烫处理)、日级为严重烫伤纽(30%TBSA，即反). A 纽假烫、B 组烫伤后 24h 经颈动脉采血分离中性辈革细胞。裂解细胞，提取蛋白质。利用周相 pH 梯度胶条(240mm X 3mm X O. 5mm , pH 3-10)Â SD5双向凝股也泳分禽蛋白质，考马斯亮蓝染色，图像扫描，PDQueSl 软件分析图像，筛 选出泛舟农达簧白质点。 再从事足股上切割差异蛋白质点，胧串串消化，应用~质辙助激光解吸/也鸡飞行时间 政谱分析，得到欣质受指纹阁，再利用 Mascot.软件搜索数据库，鉴定蛋白质。【纷来】建立了家兔外周血中性 拉细胞簧白质公DE 周培. A、日纽平均蛋白质点数分别为 (759 :七 20) 个、 (749 士 27) 个，平均匹配率分别为 93% 、 91% 。两组间筛选出 19 个差异蛋白质点，接定了其中 8 个，包括 7 种蛋白质。【结论】家兔严重烫伤后 外用血中性粒细胞蛋白质组发生改变，求达变化的蛋白质包括蛋白质二税键异构梅、ß-肌动蛋白、膜联蛋白 I 、就~特异性抗氧化蛋白等，功能涉及细胞内嗨治性、细胞能动功能及可变形性、炎症、代谢、抗氧化能力、细 胞凋1::等，这些蛋白质与严重烧伤后中性粒细胞功能改变有关。 [关键词] 烧伤s 中性命细胞: 蛋白质类; 兔 Proteome Study of Peripheral Blood Polymorphonuclear Leukocytes in Rabbits with Severe Burns ZENG J i-zhang , ZHANG Pi-hong. LI Li-li.et al ( Department 01 Plastic Surgery and Burns , Xiangya Hospítal , Omtral South University ， αangsha 410008 ， αina ) [Abstract]Objective] To study the effect of severe burns on the protein expression of polymorphonuclear leukocytes(PMNs) in rabbits. [Methods] Twelve rabbits were divided into two groups including group A (normal contro l) and group B (severe scald , 30 % TBSA ,third翩 degree). The rabbits were operated 24 hours after scald (group B) or fake scald (group A) and the peripheral blood was obtàined from the carotid artery in order to separate PMNs. The PMNs in each sample were damaged and the total proteins of cell自 were extrac- ted. The proteins were separated by 2-DE using the immobilized pH gradient and SD5. The gels were stained with coomassie brilliant blue and were scanned. The images were analyzed by PD