NF-κB及其抑制蛋白I-κBα与大肠癌侵袭和转移的相关性.pdfVIP

• 1870 中国老年学杂志2侃年8 月第29 温 NF-KB 及其抑制蛋白 I-KBQ 与大肠癌侵袭和转移的相关性 尉坤孙伟l 李秉慧I 韩梅温进坤 (河北医科大学基础医学研究所 河北省医学生物技术重点实验室，河北 石家庄 050017 ) 〔摘要〕 目的探讨NF-KB 和 I-KBa 在大肠癌中的表达特点、相互关系及其与大肠癌进展的相关性。方法 免疫组化和 Western 印迹方法 检测48 例大肠癌患者手术切除的癌组织以及配对的癌旁正常组织标本中NF唱B ， I-KBa和 p-IKßa 的表达情况，分析这3 种蛋白的表达与大肠痛侵袭 和转移的相关性。结果 大肠癌组织中 NF-KB 的表达水平明显高于正常组织(PO. 01) ，且随着肿瘤 Dukes 分期的进展，NF-KB 的表达水平呈逐渐 升高趋势。大肠癌组织中 I-KBa 的表达水平明显低于正常组织(PO.OI)，且随着肿缩Duk回分朔的进展，1-KBa 的表达水平呈逐渐降低趋势，同时， l-KBa 的磷酸化(p-IKBa) 水平逐渐升高。结论在大肠癌组织、中 I-KBa 的磷酸化降解与 NF-KB 表达升高有助于肿瘤的进展和不良顽后。 〔关键词) NF-KB;I唱Ba;大肠癌:侵袭和转移 〔申团分类号〕 曰64.3 (文献标i只码) A (文章编号) 1ω5~202(剑附)lS.1870~3 Correlations of NF.KB and I.KBαwith invasion and metastasis of colorectal cancer YU Kun , SUN Wei , LI Bing-Hui , et al. Institute of Basic Medicine , Hebei Medical University , Shijiazhuang 05ω17 ， Hebei , China [ Abstract] 0均创刊 To investigate the expressions and relationship between NF-KB and I-KBαand the correlations of them with 由e progression of colorec凶 cancer. Meth叫s The expressions of NF-KB , I-KBαand p-IKBαin cancer and normal tissues were detected by immunohistochemistry and Westem blotting. The relationship between these three proteins and the invasion and melastasis of colorectal c皿b er wωanalyzed. Res叫四e expression of NF -KB wωsignifiωntly higher in cancer tissues than normal tissues (P 0.01) ,and with the progression of Dukes stage , the level of NF -KB w臼 gradually increased. The expression of I-KBαwas lower in cancer tissues than normal tís- 5U回 (P 0.01) , and with the progr曲sion of Dukes stage , the level of I-KBαw田 gradually decreased and the leve\ of p-lKBαwas gradually increased. Conclusions Increased NF-KB r.nd reduced I-KBαis res阳nsible for the progression of colorectal cancer 皿d poor prognosis. [Key words] NF -KB; I-KBα; Colorectal cancer; Invasion and metastasis