白斑综合症病毒实时荧光LAMP 检测方法的建立及应用.pdfVIP

39 1 Vol. 39, No.1 2 0 1 5 1 ACTA HYDROBIOLOGICA SINICA Jan., 2 0 1 5 doi: 10.7541/2015.18 LAMP 1, 2 1 2 1 1 1 2 3 李红梅 江 晓 刘助红 赵 哲 任春华 胡超群 石 磊 王广军 (1. , , 510301; 2. , 510663; 3. , 510380) : ESE-Quant tube scanner , (Loop-Mediated Isothermal Amplification, LAMP), (White Spot Syndrome Virus, WSSV); , PCRReal-time PCR 4 LAMP , LAMP 63 ℃30min 105 DNA , Real-time PCR , PCR 4 LAMP ; , 5 DNA , LAMP 24 DNA, 30min66 , LAMP 7.57%, 100%, WSSV , WSSV LAMP , , , , , , , WSSV : ; LAMP; ESE-Quant tube scanner 中图分类号: Q954.4 文献标识码: A 文章编号: 1000-3207(2015)01-0142-07 (White spot syndrome virus, , , WSSV) DNA [4] , (Nimaviridae) , [5, 6] [7] [8, 9] [10] ( Whispovirus) , (ELISA) PCR PCR , 3—10d PCR[11, 12] 100%[1, 2] , WSSV , PCR, WSSV1992, ; , [3] (OIE) , (FAO)