彭泽鲫的分子遗传分析及其及方正银鲫A系的比较.pdf

　第 26 卷第 5 期 水 　产 　学 　报 Vol . 26 , N o . 5 　 2002 年 10 月 　　　　　　　　　 　　 　　　　　　 　　　　　 Oct . ,2002 　 J OURNAL OF FISHERIES OF CHINA ( ) 文章编号 : 1000 - 0615 2002 05 - 0472 - 05 ·研究简报 · 彭泽鲫的分子遗传分析及其与 方正银鲫 A 系的比较 李名友 , 　周 　莉 , 　杨 　林 , 　刘静霞 , 　桂建芳 ( 中国科学院水生生物研究所淡水生态与生物技术国家重点实验室 ,湖北 武汉 430072) 关键词 :彭泽鲫; 雌核发育银鲫; 同工酶; 转铁蛋白; 随机扩增多态性 DNA 中图分类号 :Q342 ; S917 　　　文献标识码 :A Molecular genetic analysis on Pengze crucian carp and comparison with strain A of gynogenetic silver crucian carp L I Mingyou , ZHOU Li , YAN G Lin , L IU J ingxia , GUI J ianfang ( Institute of Hy drobiology , Chinese Academy of Sciences , Wuhan 　430072 , China) Abstract : Genetic homogeneity between Pengze crucian carp and strain A of silver crucian carp was studied by using transferrin , isozyme and RA PD markers . The studied individuals of Pengze crucian carp showed transferring p atterns were the same of silver crucian carp A strain while distinct from those of other crucian carp p op ulations . As far as isozyme is concerned , the MD H , LD H and EST are all of the same with only slight differences in SOD between them . The RPAD p atterns clearly indicated high homogeneity among 16 individuals ( 6 sampled from individuals of two years old and the others aged one) from crucian carp of Pengze and 5 individuals from strain A of silver crucain carp . Nearly indentical banding p atterns were observed among all individuals . Average genetic distance within all the individuals is only 0 . 011 , suggesting crucian carp of Pe