微波辅助萃取葫芦巴茎叶挥发油工艺优化及抗氧化性研究.pdfVIP

12 0 2010, Vol. 31, No. 18 食品科学 ※工艺技术 微波辅助萃取葫芦巴茎叶挥发油工艺优化及 抗氧化性研究 王 雅，赵 萍，任海伟，张锦琇，赵丽娟 (兰州理工大学生命科学与工程学院，甘肃兰州 730050) 摘 要：采用微波辐助萃取法对葫芦巴茎叶挥发油提取工艺进行单因素和正交试验研究，用还原力、DPPH 自由 基清除能力和Ran cim at 法对其抗氧化活性进行研究。结果表明：以乙醚为萃取溶剂，从葫芦巴茎叶中萃取挥发油 的最佳工艺条件：微波功率为低火、萃取时间30s 、料液比1:4 (g/m L) 、提取前浸泡时间20m in ，在此条件下挥发 油得率达3 .374 % ；各因素对挥发油得率影响的主次顺序为料液比＞浸泡时间＞微波功率＞微波萃取时间；葫芦巴 茎叶挥发油有较强的还原能力，对DPPH 自由基有较强的清除能力，当质量浓度为200mg/L 时对猪油的抗氧化活性 最强，但其抗氧化活性小于同质量浓度的V C 。 关键词：葫芦巴茎叶；挥发油；微波；工艺优化；抗氧化性能 Microwave-assisted Extraction Optimization and Antioxidant Activity Studies of Essential Oil from the Stems and Leaves of Fenugreek WANG Y a ，ZHAO Pin g ，REN Hai-wei ，ZHAN G Jin-xiu ，ZHAO Li-j u an (School of Life Science and Engineering, Lanzhou University of Technology ，Lanzhou 730050, China) Abstract ：In the present study, we optimized the microwave-assisted diethyl ether extraction process of essential oil from the stems and leaves of fenugreek u sing single factor and orthogonal array design methods. In addition , reducing power, DPPH radical scavenging and Rancimat assays were used to see whether the extracted oil has antioxidant effect. The optimal extraction conditions were found to be: microwave power, low fire; length of extraction time, 30 s; solid/liquid ratio (g/mL), 1:4; and length of pre-extraction soaking time, 20 min. An oil yield of 3.374% was obtained under such conditions. Solid/liquid ratio had the most important effect on oil yield, followed by length of pre-extraction soaking time, microwave power and length of extraction time in decreasing order. The extracted oil had strong reducing power and DPPH radical scavenging activity and pres