评价中药体外抑菌法研究进展.PDFVIP

评价中药体外抑菌法的研究进展 1 1 1 1 2 1* 张学沛 ，朱盛山 ，刘 静 ，彭 亮 ，黄娟萍 ，蔡延渠 1. 广东药学院中药开发研究所，广东 广州 510006 2. 广州普平生物科技有限公司，广东 广州 510663 摘 要：综合评述常用中药体外抑菌评价方法及存在问题，发现评价标准未能统一，结果受主观因素影响大、体外与体内抑 菌效果存在一定差异性，未能结合中药特点选择合适的方法。不同抑菌方法对评价中药对细菌的抑制能力（抑菌环、MIC ）、 杀灭能力（MBC ），细菌对药物的耐药率等影响较大。中药体外抑菌作用虽然研究方法较多，应根据研究对象、实验目的、 具体条件等选择合适的方法，以便更加准确地评价中药的抗菌作用。 关键词：中药；体外抑菌；评价方法 中图分类号：R 文献标志码：A 文章编号：1674-6376 (20 14) 02-0-0 DOI: 10.7501/j.issn.1674-6376.2014.02. Research progress in evaluation on Chinese materia medica in vitro bacteriostasis methods 1 1 1 1 2 1 ZHANG Xue-pei , ZHU Sheng-shan , LIU Jing , PENG Liang , HUANG Juan-ping , CAI Yan-qu 1. Research and Development Institute of Chinese Materia Medica, Guangdong Pharmaceutical University, Guangzhou 510006, China 2. Guangzhou PuPing Biological Technology Co., Ltd., Guangzhou 510663, China Abstract: After the common evaluation methods of in vitro bacteriostasis by Chinese materia medica (CMM) and the existing problems were comprehensively reviewed, we found that the evaluation criterion was not unified. The results were affected by subjective factors, there were some differences in the antibacterial effect between in vitro and in vivo, and the appropriate method was chosen without combining with the characteristics of CMM. Different bacteriostatic methods had a greater influence on the evaluation of CMM inhibition of bacteria (bacteriostasis circle, MIC), ability to kill (MBC), and the resistance of bacteria to drugs, etc. There are many research methods on the bacteriostasis experiment in vitro of CMM. Therefore, suitable methods should be chosen according to the conditions, aims, and requirements, so as to evaluate the antibacterial effect of CMM more accurately. Key words: Chinese materia medica; in vitro bacteriostasis; ev