异基因造血干细胞移植后人类疱疹病毒6型感染及其与巨细胞病毒感染的相关性论文.docVIP

　　异基因造血干细胞移植后人类疱疹病毒6型感染及其与巨细胞病毒感染的相关性论文 王立茹 董陆佳 陆道培 【摘要】 为了解人类疱疹病毒6型（human herpesvirus 6, HHV-6）在中国接受异基因造血干细胞移植（hematopoietic stem cell transplantation, HSCT）的人群中感染现状及其与巨细胞病毒（cytomegalovirus, CMV）感染的相关性，对72例接受HSCT的患者HHV-6 DNA血症进行连续监测。收集HSCT患者移植前和移植后1-12周EDTA抗凝的外周血标本共680份，采用巢式聚合酶链反应检测外周血单个核细胞中HHV-6 DNA，并利用Hind Ⅲ 限制性内切酶对HHV-6进行基因分型，同时采用免疫荧光法检测CMV抗原血症。结果显示，HSCT后62.5%（45/72）的患者至少1次出现HHV-6 DNA血症，首次检出的中位时间为14（7-63）天；除1例患者检出HHV-6A型以外，其余所有HHV-6阳性患者均为HHV-6B型感染。65.3%（47/72）的移植后患者至少1次发生CMV抗原血症.freelan Herpesvirus-6 in Allogeneic Hematopoietic Stem Cell Transplant Recipients in Correlation egalovirus Infection Abstract In order to study the prevalence of human herpesvirus 6 (HHV-6) in allogeneic hematopoietic stem cell transplant (allo-HSCT) recipients in China and to analyze the relationship betegalovirus (CMV) infection in post-HSCT patients, nested polymerase chain reaction (PCR) onitor HHV-6 DNAemia in 72 consecutive allo-HSCT recipients. 680 EDTA anticoagulated peripheral blood specimens ia munofluresence stain. The results shoia edian day 14 (range, 7-63 days) after allo-HSCT, and HHV-6B inant variant. CMV antigenemia edian day 43 (range, 14-105 days) after allo-HSCT. Co-infection of HHV-6 and CMV (HHV-6+/CMV+) occurred in 52.8% (38/72) recipients. The onset of HHV-6 DNAemia ia (P 0.0001). Patients ia positive ore likely to have concurrent CMV antigenemia than HHV-6 DNAemia negative patients (84.4% vs 33.3%, P=0.0001) after allo-HSCT. Among the herpesvirus related disease, the relatively high incidence of hemorrhage cystitis (HC) occurred in 23.6% (17/72) of post-HSCT patients. 88.2% (15/17) of HC developed in HHV-6 positive patients，and 82.3% (14/17) occurred in CMV+/HHV-6+ patients. It is concluded that infection of HHV-6, co-infection of HHV-6 and CMV, monly occurred in post-HSCT patients in China,.freelia. Key atopoietic stem cell transplantation; human herpesvirus 6; cytomegalovirus 人类疱疹病毒6型（human herpesvirus 6, HHV-6）与巨细胞病毒（cytomegalovirus, CMV）同属于β疱疹病毒亚家族。根据基因组