响应面优化超声辅助提取大豆异黄酮联产分离蛋白和低聚糖.pdfVIP

第 31 番第4 期 大豆科学 Vo l. 31 No .4 2012 年 8 月 SOYBEAN SCIENCE Aug. 2012 晌应面优化超声辅助提取大豆异黄嗣联产分离蛋白和fli 聚糖 郭建，王梦凡，齐威，苏荣欣，何志敏 (天津大学化工学院化学工程研究所，天津 30田72) 摘 要设计了一条绿色高效地提取纯化大豆异黄酬，并联产制备大豆分离蛋白和低聚糖的工艺。首先通过单因素 实验和响应面(RSM) 优化，确定了超声辅助提取大豆异黄嗣的工艺条件:超声功率250 W， 70% 乙酶，提取温度 70C , 提取时间 120 min ，大豆异黄酬提取量为 2 104.25 罔-g i ，提取率85.34% ;并通过三步简单的分离纯化从豆粉中得到 1 838. ∞问-t1 、纯度为 62.099毛的异黄酬，最终收率 74.54% ;最后以提取异黄嗣后的剩余豆渣为原料，联产制备了 大豆分离蛋白(216.25 mg.g-) 和低聚糖(73.75 mgg 勺，全过程无废液废渣排出。 关键词:大豆异黄嗣;晌应面优化;提取;纯化;综合利用 中图分类号:TS218 文献标识码:A 文章编号:1∞0-9841 (2012 )04()655()7 Optimization of UltrasonÏc -assisted Extraction of Soybean Isoflavones by Re- sponse Surface Methodology and Simultaneous Preparation of Protein Isolates: and Oligosaccharides GUO Jian ,WANG Meng-fan ,QI W凹，SU Rong-xin ,HE Zhl-min • (Chemical Engineering R四earch Center ,School of Chemical Engineering and Technology ,Tianjin Univenity ,Tianjin 3αXJ72 ， Chlina ) Abstract: An effective and 伊en strategy was developed 10 obtain isofl_ avones from soybean. 50y protein isolates 皿d oligosac charides were also obtained from soybean dregs simultaneously. Firstly , the optimal extraction conditions were investigated by single factor experiments and RSM optimization. A high extraction amount (2 104. 25 j.L g g - 1 ) and yield ( 85. 34% ) of Îsofla- vones were achieved under the optimal extraction eonditions: ultrasonic power of 250 W ,70% ethanol ,70 C ,120 min. Second- ly ,the purified isof1 avones of 1 838.00 问-g l W醋。htained with purity of 62.09% and recove可 of 74. 54% via three simple purification steps. Finally ,the amount of soy protein isolates and oligosaccharides obtained from soybean dregs achieved 216. 25 1 mg.